HPLC-ELSD法测定阿米卡星洗剂的含量___
HPLC-ELSD analysis of amikacin lotion
分类号：
出版年·卷·期（页码）：2009,29 (6):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
摘要 目的：建立高效液相色谱－蒸发光散射检测器（HPLC-ELSD）检测法测定阿米卡星洗剂的含量。方法：采用Agilent ZORBAX Extend-C18（4.6 mm×250 mm，5 μm）碱性色谱柱，流动相为水-氨水-冰醋酸（96∶3.6∶0.4，用氨水或冰醋酸调节pH至10.0)，流速为1.0 mL•min-1，漂移管温度为50 ℃，雾化气体压力为3.5×105 Pa。结果：阿米卡星和主要杂质K29分离良好。阿米卡星在0.6~6.0 mg•mL-1的浓度范围内呈较好的线性关系（r=0.9999），阿米卡星的平均回收率（n=9）为100.2%。结论：方法快速、简便，结果准确可靠，重复性好。
-----英文摘要：---------------------------------------------------------------------------------------
Abstract Objective:To develop HPLC-ELSD method for the determination of amikacin lotion.Methods:The column was Agilent ZORBAX Extend-C18（4.6 mm×250 mm，5 μm）.The mobile phase was water-ammonia water-glacial acetic acid（96∶3.6∶0.4,adjust pH to 10.0 with ammonia or acetic acid)at a flow rate of 1.0 mL•min-1.The drift tube temperature was 50 ℃.The pressure of nebulizing gas was 3.5×105 Pa.Results:Good separation of amikacin and main relate substance K29 could be achieved.The standard curve was rectilinear in the range of 0.6-6.0 mg•mL-1（r=0.9999）.The average recovery（n=9） was 100.2%.Conclusion:The method is simple and rapid,the results are accurate and reproducible.
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