RP-HPLC同时测定葛根异黄酮提取部位中葛根素、大豆苷、染料木苷和大豆苷元的含量___

 
摘要 目的：建立控制葛根异黄酮提取部位的定性和定量分析方法。方法：用反相高效液相色谱法(RP-HPLC)，Kromasil KR100-5 C18(4.6 mm×250 mm,5 μm)柱，流动相为乙腈-0.03 mol•L-1醋酸铵，梯度洗脱，流速0.7 mL•min-1，检测波长250 nm，柱温30 ℃，对葛根异黄酮部位中的主要成分葛根素、大豆苷、染料木苷、大豆苷元进行定量测定。结果：色谱峰分离情况良好，葛根素、大豆苷、染料木苷、大豆苷元可达到定性、定量要求。葛根素在0.13~0.66 μg、大豆苷在0.03~0.14 μg、染料木苷在0.03~0.16 μg、大豆苷元在0.02~0.12 μg范围内，线性良好(r>0.9995)，平均回收率为98.1%~99.3%。结论：所建方法准确、简便，重复性好，可用于葛根异黄酮部位中主成分的定性、定量考察。

Abstract Objective:To develop a quantitative determination of puerarin,daidzin,genistin,daidzein in the fraction extracted of pueraria isoflavone.Methods:The samples were analyzed on a Kromasil KR100-5 C18(4.6 mm×250 mm,5 μm) column with acetonitrile-0.03 mol•L-1 ammonium acetate as a mobile phase using a gradient elution.The flow rate was 0.7 mL•min-1,and UV detection wavelength was set at 250 nm.Results:The linear ranges of puerarin,daidzin,genistin,daidzein were 0.13-0.66 μg,0.03-0.14 μg,0.03-0.16 μg,0.02-0.12 μg,respectively(r﹥0.9995).The average recoveries were 98.1%-99.3%.Conclusion:The new method is fast,accurate,sensitive,selective,and suitable for use in the quality control of pueraria isoflavone fraction

1ZHANG Yan（张岩）,XU Qing（徐青）,XUE Xing-ya(薛兴亚), et al.Construction of component library of Kudzu root(葛根组分库的构建).World Sci Technol Mod Tradit Chin Med (世界科学技术-中医药现代化),2005,7(6):62
2LIANG Gui-xian(梁桂贤),LI Qin(李勤).Development of method for the extraction separation and study on pharmaceutics of flavonoids puerariae(葛根黄酮提取分离方法及制剂研究进展).J Shanxi Coll Tradit Chin Med (山西中医学院学报),2001,2(4):47
3JIN Wen-shan(金文姗),TAN Yu-yuan(谈钰元),CHEN You-gen(陈有根).Determination of puerarin,daidzin and daidzein in root of Pueraria lobata of different origin by HPLC(高效液相色谱法测定不同产地葛根中葛根素、大豆苷及大豆苷元的含量).China J Chin Mater Med (中国中药杂志),2003，28(1):49
4ZHU Li-hua(朱丽华),HE Lang-chong(贺浪冲).Qualitative and quantitative analysis of the effective part and effective components of Radix Puerariae(葛根中有效部位及有效成分的高效液相色谱分析).J Xian Jiaotong Univ(Med Sci)(西安交通大学学报 医学版),2005,26（3):216