RP-HPLC法测定罗布麻叶中金丝桃苷和罗布麻甲素的含量___
RP-HPLC analysis of hyperoside and isoquercitrin in Apocynum venetum L.
分类号：
出版年·卷·期（页码）：2009,29 (6):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
摘要目的:建立测定罗布麻叶中金丝桃苷和罗布麻甲素含量的方法。方法：采用Zorbax-C18(4.6 mm×150 mm，5 μm)色谱柱，以乙腈-1.0%醋酸溶液(14∶86)为流动相，流速0.8 mL•min-1，检测波长360 nm，柱温30 ℃。结果：金丝桃苷和罗布麻甲素的线性范围分别为0.024~0.487 μg和0.028~0.551 μg 平均回收率(n=9)分别为98.7%和99.8%。结论：所建立的HPLC法可用于同时测定罗布麻叶中金丝桃苷和罗布麻甲素的含量。
-----英文摘要：---------------------------------------------------------------------------------------
Abstract Objective:To establish the quantitative analysis of hyperoside and isoquercitrin by RP-HPLC.Methods:The determination of hyperoside and isoquercitrin was conducted by RP-HPLC using a Zorbax-C18(4.6 mm×150 mm,5 μm)column.The mobile phase was acetonitrile-1.0% acetic acid solution(14∶86) with the flow rate of 0.8 mL•min-1,the detection wavelength was 360 nm,and the column temperature was set at 30 ℃.Results:In HPLC analysis,the linear ranges of hyperoside and isoquercitrin were 0.024-0.487 μg and 0.028-0.551 μg,respectively The average recoveries(n=9) were 98.7% and 99.8%,respectively.Conclusion:The established method can be used to determine the contents of hyperoside and isoquercitrin in Apocynum venetum L.simultaneously.
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