RP-HPLC法同时测定天麻头风灵胶囊中梓醇和天麻素的含量___
RP-HPLC simultaneous determination of catalpol and gastrodin in Tianma Toufengling capsules
分类号：
出版年·卷·期（页码）：2009,29 (6):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
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摘要 目的：建立RP-HPLC法同时测定天麻头风灵胶囊中梓醇和天麻素的含量。方法：采用Diamonsil C18(200 mm × 4.6 mm,5 μm)色谱柱 流动相为乙腈-0.1%磷酸溶液(2∶98,v/v) 流速1.0 mL·min-1 柱温 35 ℃ 检测波长 210 nm。结果：梓醇和天麻素的浓度分别为4.60~46.0 μg·mL-1和18.6~186 μg·mL-1,相关系数分别为0.9997和0.9998 平均回收率(n=9)分别为101.4%和101.0%。结论：本方法简便、准确，重复性好，可用于天麻头风灵胶囊的质量控制。
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Abstract Objective:To develop an RP-HPLC method for the simultaneous determination of catalpol and gastrodin in Tianma Toufengling capsules.Methods:The chromatographic separation was achieved on a Diamonsil C18(200 mm × 4.6 mm,5 μm) column with a mobile phase of acetonitrile-0.l% phosphoric acid solution(2∶98,v/v).The flow rate was 1.0 mL·min-1 and the column temperature was 35 °C.Results:The calibration curve was linear within the range of 4.60-46.0 μg·mL-1(r=0.9997) for catalpol and 18.6-186 μg·mL-1(r=0.9998) for gastrodin,respectively.The recoveries(n=9) of catalpol and gastrodin were 101.4% and 101.0%,respectively.Conclusions:The method is accurate,sensitive and specific.It can be used for the quality control of Tianma Toufengling capsules.
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