HPLC法测定白花前胡甲素在大鼠肝微粒体中的含量___
HPLC determination of praeruptrum A(Pd-Ia) in rat hepatic microsomal fraction
分类号：
出版年·卷·期（页码）：2009,29 (6):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
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摘要 目的：建立一种快速准确检测大鼠肝微粒体中白花前胡甲素（Pd-Ia）含量的方法。方法：以华蟾毒精为内标物，采用Hypersil-C18(250 mm × 4.6 mm,5 μm)色谱柱，以甲醇-水(75∶25)为流动相，流速1 mL·min-1，检测波长321 nm,柱温25 ℃，进样量20 μL。结果：Pd-Ia浓度在 1~100 μg·mL-1范围内与峰面积呈现良好的线性关系（r=0.9989）日内精密度RSD≤2.9% 日间精密度RSD≤8.5% 平均加样回收率(n=9)为98.4%。结论：本文建立的方法操作简单，可用于Pd-Ia含量的测定。
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-----英文摘要：---------------------------------------------------------------------------------------
Abstract Objective:To establish a method of determination of praeruptrum A(Pd-Ia)in rat hepatic microsomes fraction by HPLC.Method：Hypersil-C18(250 mm × 4.6 mm,5 μm) column was adopted at a temperature of 25 ℃.An HPLC method was established using cinobufagin as internal standard.The mobile phase consisted of methanol-water (75∶25) at a flow rate of 1.0 mL·min-1.The UV detection wavelength was set at 321 nm,the injection volume was 20 μL.Results:The calibration curve was shown to be a linear over the range of 1-100 μg·mL-1 (r=0.9989）.The mean recovery(n=9) was 98.4%,The RSDs of intra-day and inter-day were less than 2.9% and 8.5%,respectively.Conclusion:The method is specific,simple and has been successfully applied to the determination of Pd-Ia.
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