HPLC法测定复方救心片中人参皂苷Rg1、Re的含量___
HPLC determination of ginsenoside Rg1,Re in compound Jiuxin tablets
分类号：
出版年·卷·期（页码）：2009,29 (5):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
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摘要 目的：建立高效液相色谱法测定复方救心片中人参皂苷Rg1、Re含量。方法：采用SUPELCO C18柱(150 mm×4.6 mm，5 μm)，流动相为乙腈-0.06%磷酸溶液(100∶400)，流速：0.8 mL·min-1，检测波长203 nm，柱温30 ℃。结果：人参皂苷Rg1进样量在2.18～10.90 μg(r=0.9999)范围内线性关系良好，平均回收率(n=6)为99.85%(RSD=1.5%)；人参皂苷Re进样量在2.21～11.05 μg(r=0.9999)范围内线性关系良好，平均回收率(n=6)为98.79%(RSD=2.3%)。结论：本方法操作简便，结果准确可靠，重复性好。
关键词：复方救心片；人参皂苷Rg1、Re；高效液相色谱法
-----英文摘要：---------------------------------------------------------------------------------------
Abstract Objective:To establish an HPLC method for the determination of ginsenoside Rg1,Re in compound Jiuxin tablets.Method:The SUPELCO C18 column(150 mm×4.6 mm，5 μm) was adopted;The mobile phase was a mixture of acetonitrile-0.06% phosphoric acid solution(100∶400) and the flow rate was 0.8 mL·min-1;The detection wavelength was 203 nm and column temperature was controlled at about 30 ℃.Results:For ginsenoside Rg1,the method had good linear relationship within the range of 2.18 to 10.90 μg with the correlation coefficient 0.9999,and the average recovery(n=6) was 99.85% with RSD of 1.5%;For ginsenoside Re,the method had good linear relationship within the range of 2.21 to 11.05 μg with the correlation coefficient 0.9999,and the average recovery(n=6) was 98.79% with RSD of 2.3%.Conclusion:The method is simple, accurate, sensitive and has good reproduction.
Key words:compound Jiuxin tablets;ginsenoside Rg1,Re;HPLC
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