丹参酮Ⅱ_A磺酸钠在家兔体内的药代动力学研究___

Abstract  Objective：To establish an RP-HPLC method for the determination of sodium tanshinone Ⅱ_A sulfonate in rabbits plasma.Method:Protein precipitation with acetonitrile was applied to purify plasma samples before RP-HPLC analysis.The content of sodium tanshinone Ⅱ_A sulfonate in plasma samples was determined on Hypersil C₁₈ column(250 mm×4.6 mm,10 μm)with KH₂PO₄(0.01 mol·L^-1),with methyl alcohol and triethylamine(33∶67∶05,adjusted to pH 5.5± 0.1 with phosphoric acid)as the mobile phase,flow rate of 1 mL·min^-1,detected at 271 nm and column temperature at room temperature.Result:The retention time of sodium tanshinone ⅡA sulfonate was observed at 6.6 min.The linear range of calibration curve was within drug concentrations of 0.1-30 mg·L^-1(n=5,r=0.9994).The quantitation limit was 0.1 mg·L^-1.The analytical recovery was more than 95%,intra-and inter-day precision RSDs were all less than 5.0%(n=5).The experimental data showed that the concentration-time curve of sodium tanshinone Ⅱ_A sulfonate in rabbits plasma could be fitted to three-compartment model(W＝1).Conclusion:These findings can provide references during pharmacokinetics investigations in humans for sodium tanshinone Ⅱ_A sulfonate．

Key words:sodium tanshinone Ⅱ_A sulfonate;sodium tanshinone Ⅱ_A sulfonate for injection;HPLC;pharmacokinetics;rabbits