黄芪中异黄酮类成分的研究___

Abstract   Objective：To corroborate the structures of the four isoflavonoids(calycosin-7-O-β-D-glucopyranoside,formononetin-7-O-β-D-glucopyranoside,calycosin and formononetin)in Radix Astragali by LC-MS/MS method and compare their relative contents in Radix Astragali from different sources.Methods:HPLC-DAD analysis was performed with a Waters 2695 high performance liquid chromatogram,equipped with a Waters 2996 diode array detector.LC-MS/MS analysis was performed with an Agilent G6300 serious LC-MSD Trap.The separation was carried out by using an Alltima C₁₈ column(250 mm×4.6 mm,5 μm) with a gradient mobile phase of acetonitrile-water solution,which column temperature,flow rate and detector wavelength were set at 35 ℃,0.8 mL·min^-1 and 260 nm.The analytes were eluted within 90 min.Results:The LC-MS/MS technique could be applied to the qualitative analysis of the chemical component in the same type.There was difference in the content of four isoflavonoids from Radix Astragali of different sources.Conclusion:The developed method here is useful to control the quality of Radix Astragali,and distinguish Radix Astragali from the other samples by the simultaneously qualitative analysis of the four isoflavonoids.

Key words:HPLC-ESI-MS/MS;isoflavonoid;Radix Astragali