短小棒状杆菌免疫增强机理的初步研究___

摘要：尝试经超生破碎、蛋白酶K消化、CTAB沉淀及酚氯仿抽提等步骤从短棒杆菌（Corynebacterium parvum，CP）中提取基因组DNA（CP-CpG-DNA），结果显示提取的基因组DNA比较纯净，完整性好。提取的基因组大小范围为1000-23130bp，主要集中在2.3Mb。CP-CpG-DNA对HpaII高度敏感，被酶切成小于1000bp的片断，显示所制备的CP-CpG-DNA含有较多的未甲基化的CpG基序，提示CP-CpG-DNA具有潜在的免疫增强的功能，可用于开发基于CP-CpG-DNA的DNA免疫增强佐剂。

Abstract: Corynebacterium parvum (CP), together with Mycobacterium bovis bacillus Calmette-Guérin (BCG), is one of the most widely used biological adjuvants for activating primate immune response. High quality of CP genome DNA (designated as CP-CpG-DNA) were prepared from the CP strain suspension by a series of treatments including sonification, protease k digestion, CTAB precipitation, and premixed phenol:chloroform:isoamyl alcohol extraction. The extracted CP-CpG-DNA has a molecular weight ranging from 1000-23130bp with a main band at about 23130bp. The CP-CpG-DNA can be digested by HpaII enzyme to little fragments of less than 1000bp in length, demonstrating hypersensitivity to HpaII, indicating high quantities of unmethylated CpG motifs in CP-CpG-DNA. Results give us a sign that CP-CpG-DNA could be used as a potential immunity enhancer.