棒束孢属菌(Isaria_sp.)中游离尿苷、腺嘌呤、腺苷的HPLC测定___
HPLC Determination of Uridine,Adenine and Adenosine in the Mycelia of Isaria sp.
分类号:
出版年·卷·期(页码):1996,16 (4):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------
本文用Waters HPLC系统、Radial-PakTM C18径向加压柱、甲醇—0.01mol/L磷酸二氢钾(10∶90)为流动相定量测定了虫草菌—棒束孢菌丝体中游离尿苷、腺嘌呤、腺苷的含量。检测波长为254nm,方法平均回收率90.6%~93.1%,相对标准偏差0.92%~1.58%(n=6)。方法简便、灵敏度高。
-----英文摘要:---------------------------------------------------------------------------------------
A method for the determination of uridine,adenine and adenosine in the mycelia of Isaria sp.by HPLC was described.On Radial-PakTM C18 column using methanol-0.01mol/L KH2PO4 as mobile phase and detected at 254nm,the standards and sample could be separated
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