白杨素衍生物抗氧化活性的研究

目的： 以人正常肝细胞株（L-O2细胞）和昆明小鼠为实验对象，研究白杨素衍生物（CD）的抗氧化活性。方法： 分别用不同浓度CD（2.5、5、10、20、25 μmol·L^-1）作用L-O2细胞24 h后，采用MTT法检测细胞活力。将L-O2细胞分对照组、H₂O₂模型组和CD治疗组，采用ELISA法检测CD对H₂O₂损伤的L-O2细胞中谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、过氧化物酶（CAT）活性和丙二醛（MDA）含量的影响。昆明小鼠随机分为对照组、H₂O₂模型组和CD治疗组（5、10 mg·kg^-1），实施腹腔注射药品，眼眶取血，随后断头处死小鼠，取肝脏组织。检测小鼠血液和肝脏组织中GSH-Px、SOD、CAT的活性及MDA含量。结果： CD的浓度为2.5~5.0 μmol·L^-1时，对L-O2细胞的活力无显著影响，因此选择2.5 μmol·L^-1作为CD的保护浓度。与H₂O₂模型组相比，CD治疗组中L-O2细胞的GSH-Px、SOD、CAT的活性显著上升，丙二醛含量显著下降；小鼠给药浓度为5 mg·kg^-1时，与模型组相比，CD治疗组小鼠血液中GSH-Px、SOD和CAT活性分别升高了108.41%、54.00%、74.29%，丙二醛含量下降47.01%。小鼠肝脏组织中GSH-Px、SOD和CAT活力比模型组分别增加了44.91%、45.17%和55.21%，同时丙二醛含量下降了54.00%。结论： CD对H₂O₂损伤的L-O2细胞和组织具有保护作用，且表现为浓度依赖性，其潜在机制可能是提高细胞和组织抗氧化能力，从而减轻不良氧化应激反应。

Objective: To investigate the antioxidant activities of chrysin derivative(CD)by using the L-O2 cell line and KM mice. Methods: L-O2 cells were treated with different concentrations of CD(2.5,5,10,20, 25 μmol·L^-1)for 24 h,and the cell viability was measured using MTT assay. L-O2 cells were divided into control group,H₂O₂ model group and CD treatment groups. The activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),catalase(CAT)and the level of malondialdehyde(MDA) in L-O2 cells were detected by ELISA. KM mice were randomly divided into control group,H₂O₂ model group and CD treatment groups(5 mg·kg^-1,10 mg·kg^-1)and were administrated with the control or CD at different concentrations intraperitoneally. Blood was collected from orbital and the liver tissues were collected after r decapitation. The activities of GSH-Px,SOD,GSH-Px,CAT and the level of MDA in both blood and liver tissues were detected by ELISA. Results: CD at the concentrations from 2.5~5.0 μmol·L^-1 showed no significant effect on the viability of L-O2,therefore 2.5 μmol·L^-1 was used as the protective concentration of CD. Compared with the H₂O₂ model group,the activities of GSH-Px,SOD,CAT of L-O2 cells in the CD treated groups increased significantly,while the level of MDA was significantly decreased. When the dosage for the mice was 5 mg·kg^-1,comparing with the model group,the activities of GSH-Px,SOD,CAT in CD-treated group mice blood increased by 108.41%,54.00% and 74.29%,respectively,while the level of MDA decreased by 47.01%. The activities of GSH-Px,SOD and CAT in mice liver tissues increased by 44.91%,45.17% and 55.21%,respectively,and the level of MDA decreased by 54.00%. Conclusion: CD has a protective effect on H₂O₂-injured L-O2 cells and tissues in a dose-dependent manner,and the underlying mechanism may be the increasing of antioxidant capacity of cells and tissues,thereby reducing the adverse oxidative stress.

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