基于UPLC-MS的黄芪药材质量评价研究

目的： 建立超高效液相色谱-三重四极杆质谱串联法（UPLC-TQ-MS法）同时测定不同产地黄芪中17个成分（异黄芪皂苷Ⅳ，黄芪皂苷Ⅳ，黄芪皂苷Ⅲ，黄芪皂苷Ⅱ，大豆皂苷Ⅰ，异黄芪皂苷Ⅱ，黄芪皂苷Ⅰ，异黄芪皂苷Ⅰ，毛蕊异黄酮苷，染料木苷，芒柄花苷，美迪紫檀苷，毛蕊异黄酮，染料木素，芒柄花素，3-羟基-9，10-二甲氧基紫檀烷，7，2-二羟基-3，4-二甲氧基异黄烷）的含量，分析各成分之间的相关性并利用主成分分析与方差分析比较不同产地黄芪的差异。方法： 采用Acquity UPLC BEH C₁₈色谱柱（100 mm×2.1 mm，1.7 μm），流动相为0.1%甲酸水溶液-乙腈，梯度洗脱，流速0.35 mL·min^-1，柱温35℃，采用多反应监测方法进行质谱扫描。结果： 17个待测成分的浓度与峰面积线性关系良好（0.992 7 < R² < 0.999 1）。相关性分析结果显示8个皂苷之间具有显著正相关，9个黄酮之间具有显著正相关。主成分分析的F综合评分结果显示宁夏产黄芪质量较优。方差分析结果显示黄芪皂苷Ⅰ、染料木素、芒柄花素、3-羟基-9，10-二甲氧基紫檀烷、7，2-二羟基-3，4-二甲氧基异黄烷在宁夏产黄芪中含量最高，毛蕊异黄酮在宁夏、山西产黄芪中含量较高。结论： 建立的黄芪药材质量评价方法准确可靠，可为黄芪药材质量控制提供参考依据。

Objective: To establish an UPLC-TQ-MS method for simultaneous determination of 17 components(isoastragaloside Ⅳ,astragalosideⅣ,astragalosideⅢ,astragaloside Ⅱ,soyasaponin Ⅰ,isoastragaloside Ⅱ, astragaloside Ⅰ,isoastragalosideⅠ,calycosin-7-O-β-D-glucoside,genistein 7-O-β-D-glucoside,ononin, methylnissolin-3-O-glucoside,calycosin,genistein,formononetin,medicarpin and isomucronulatol)in Astragali Radix from different habitats.And correlation analysis was used to analyze the correlation among the components.At the same time,principal component analysis and variance analysis were used to compare the differences of Astragali Radix from different habitats.Methods: Chromatographic separation was carried out on Acquity UPLC BEH C₁₈ column(100 mm×2.1 mm,1.7 μm)with gradient elution of 0.1% formic acid-acetonitrile at a flow rate of 0.35 mL·min^-1.The column temperature was 35℃ and the multiple reaction monitoring scan was adopted.Results: The concentration of 17 tested components had a good linear relationship with peak area (0.992 7 < R² < 0.999 1).Correlation analysis showed that there was a significant positive correlation between 8 saponins and there was a significant positive correlation between 9 flavonoids.The results of F comprehensive score of principal component analysis showed that the quality of Astragali Radix from Ningxia was better.The results of ANOVA showed that the contents of astragaloside Ⅰ,genistein,formononetin,medicarpin and isomucronulatol in Ningxia Astragali Radix were higher and the contents of calycosin in Ningxia and Shanxi Astragali Radix were higher.Conclusion: The established quality evaluation method of Astragali Radix medicinal materials is accurate and reliable,which can provide a reference for the quality evaluation of Astragali Radix medicinal materials.

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