维药驱虫斑鸠菊化学成分、质量控制及其药理活性的研究进展

维药驱虫斑鸠菊为菊科斑鸠菊属植物驱虫斑鸠菊（Vernonia anthelmintica Willd.）的干燥成熟种子，是维吾尔族常用药材，主产于中国新疆和巴基斯坦等地。由于其对于白癜风等皮肤病的治疗有独特的疗效，近年来受到越来越多的关注。驱虫斑鸠菊中主要化学成分为倍半萜及其二聚体、黄酮、甾体等类成分。由于产地独特的地理环境和气候，形成了较为独特的化学成分，倍半萜类成分主要为榄香烷型，该类型倍半萜在菊科其他属中较为少见；并且大多数分离得到的倍半萜在8位上连接有较为少见的2-（羟甲基）丙烯酰基。驱虫斑鸠菊的质量控制及药理活性也有报道。本文对驱虫斑鸠菊的化学成分、质量控制及药理活性进行系统归纳整理，以期为该药材的开发利用提供参考。

Fructus Vernoniae is dry mature seeds of Vernonia anthelmintica Willd. belonging to Compositae. It is frequently used as medicinal herbs in the Uyghur medicines and is mainly produced in Xinjiang Uygur aotonomous region and Pakistan. Due to its unique curative effect on the treatment of skin diseases such as vitiligo,it has received more and more attention in recent years. The main chemical constituents of V. anthelmintica are sesquiterpenoids and their dimers,flavonoids and steroids. Due to the unique geographical environment and climate of the place of origin,relatively unique chemical constituents were produced. Most sesquiterpenoids are elemanolides,which are rare in othergenus of Compositae. And a relatively rare 2-(hydroxymethyl) acryloyl group is attached to 8-position of elemanolide. There were also many reports on quality control and pharmacological activities of V. anthelmintica. This paper summarized chemical constituents,quality control and pharmacological activities of this herb with expectation to provide valuable reference for development and utilization of this herb.

[1] 中国科学院中国植物志编辑委员会.中国植物志.第74卷[M].北京:科学出版社,1985:74 Editorial Committee of Chinese Journal of Plant of Chinese Academy of Sciences.Flora of China.Vol 74[M].Beijing:Science Press,1985:74
[2] 卫生部药品生物制品检定所.中国民族药志[M].北京:人民卫生出版社,1984:312 Institutes for the Control of Pharmaceutical and Biological Products,Ministry of Health,PR China.Records of Chinese Ethnic Medicines[M].Beijing:People's Medicical Publishing House,1984:312
[3] 蔡良谟,霍仕霞,林娟,等.维药驱虫斑鸠菊化学成分研究[J].中成药,2012,34(11):2159 CAI LM,HUO SX,LIN J,et al.Chemical constituents of Vernonia anthelmintica (L.) Willd.[J].Chin Tradit Pat Med,2012,34(11):2159
[4] LIU Y,NUGROHO AE,HIRASAWA Y,et al.Vernodalidimers A and B,novel orthoester elemanolide dimers from seeds of Vernonia anthelmintica[J].Tetrahedron Lett,2010,51(50):6584
[5] ZHANG L,SHAO L,HUA L,et al.Guaianolides and elemanolides from Vernonia anthelmintica[J].Phytochem Lett,2014,7(1):14
[6] TURAK A,LIU Y,AISA HA.Elemanolide dimers from seeds of Vernonia anthelmintica[J].Fitoterapia,2015,104:23
[7] ITO T,AIMAITA S,WIN NN,et al.New sesquiterpene lactones,vernonilides A and B,from the seeds of Vernonia anthelmintica in Uyghur and their antiproliferative activities[J].Bioorg Med Chem Lett,2016,26(15):3608
[8] TURAK A,MAIMAITI Z,MA HR,et al.Pseudo-disesquiterpenoids from seeds of Vernonia anthelmintica and their biological activities[J].Phytochem Lett,2017,21:163
[9] TURAK A,AISA HA.Three new elemanolides from the seeds of Vernonia anthelmintica[J]. J Asian Nat Prod Res,2018,20(4):313
[10] 王加利,潘纪红,袁将,等.驱虫斑鸠菊化学成分及酪氨酸酶抑制活性研究[J].中药材,2017,40(8):1833 WANG JL,PAN JH,YUAN J,et al.Chemical constituents and tyrosinase inhibition activities of the fruits from Vernonia anthelmintica[J].J Chin Med Mater,2017,40(8):1833
[11] TIAN G,ZHANG U,ZHANG T,et al.Separation of flavonoids from the seeds of Vernonia anthelmintica Willd.by high-speed counter-current chromatography[J].J Chromatogr A,2004,1049(1):219
[12] MAIMAITA Z,TURAK A,AISA HA.Two new compounds from the seeds of Vernonia anthelmintica[J].J Asian Nat Prod Res,2017,19(9):862
[13] HUA L,QI WY,HUSSAIN SH,et al.Highly oxygenated stigmastane-type steroids from the aerial parts of Vernonia anthelmintica Willd.[J].Steroids,2012,77(7):811
[14] HUA L,LI Y,WANG F,et al.Biologically active steroids from the aerial parts of Vernonia anthelmintica Willd.[J].Fitoterapia,2012,83(6):1036
[15] 花蕾.四种药用植物化学成分及其生物活性研究[D].兰州:兰州大学,2012 HUA L.Studies on the Chemical Constituents and Their Bioactivities of Four Medicinal Plants[D].Lanzhou:Lanzhou University,2012
[16] 吴剑飞,沙也夫·木沙德林,冯胜初,等.驱虫斑鸠菊化学成分的研究[J].化学学报,1991,49(10):1018 WU JF,SHAYEFU MSDL,FENG SC,et al.Study on chemical constituents of Vernonia anthelmintica[J].Acta Chim Sin,1991,49(10):1018
[17] 曾文浩,屈保平,张星平,等.驱虫斑鸠菊治疗白癜风研究进展[J].中医药学报,2016,44(2):96 ZENG WH,QU BP,ZHANG XP,et al.Research advances of treating vitiligo with Vernonia anthelmintica[J].Acta Chin Med Pharmacol,2016,44(2):96
[18] 孙华梅,许子涛.驱虫斑鸠菊注射液结合白癜风丸治疗白癜风疗效观察[J].世界最新医学信息文摘,2015(86):185 SUN HM,XU ZT.Therapeutic effect of Vernonia anthelmintica injection combined with vitiligo pill on vitiligo[J].World Latest Med Inform,2015(86):185
[19] 梁国越.驱虫斑鸠菊注射液联合复方卡力孜然酊治疗白癜风疗效观察[J].中国医学文摘(皮肤科学),2011,28(4):211 LIANG GY.Therapeutic effect of Vernonia anthelmintica injection combined with compound kaliziran tincture on vitiligo[J].China Med Abst(Dermatol),2011,28(4):211
[20] 张大雷,曹冰青,郝伟丽,等.驱虫斑鸠菊注射液局封联合卤米松乳膏治疗白癜风疗效观察[J].皮肤病与性病,2014,36(1):37 ZHANG DL,CAO BQ,HAO WL,et al.Therapeutic effect of Vernonia anthelmintica injection combined with halogenat asone cream on vitiligo[J].J Dermatol Venereol,2014,36(1):37
[21] 吴波,朱颜俊,顾兰秋,等.308 nm准分子激光联合驱虫斑鸠菊注射液治疗白癜风60例临床观察[J].中国皮肤性病学杂志,2014,28(11):1209 WU B,ZHU YJ,GU LQ,et al.Efficacy of 308 nm excimer laser plus Vernonia anthelmintica Willd. (VW) injection in 60 cases of vitiligo[J].Chin J Dermatovenereol,2014,28(11):1209
[22] 赵萍萍,霍仕霞,闫明,等.驱虫斑鸠菊提取物指纹图谱与其对人A375黑素瘤细胞增殖作用的灰关联度分析[J].中国中药杂志,2012,37(5):585 ZHAO PP,HUO SX,YAN M,et al.Grey relational analysis on fingerprint of Vernonia anthelmintica extracts and its effect on proliferation of A375 human melanoma cells[J].China J Chin Mater Med,2012,37(5):585
[23] ZHOU J,SHANG J,PING FF,et al.Alcohol extract from Vernonia anthelmintica (L.) Willd. seed enhances melanin synthesis through activation of the p38 MAPK signaling pathway in B16F10 cells and primary melanocytes[J].J Ethnopharmacol,2012,143(2):639
[24] 赵萍萍,霍仕霞,闫明,等.驱虫斑鸠菊中紫铆素、紫铆花素的分离及其对人A375黑素瘤细胞黑素合成作用的影响[J].中国药学杂志,2013,48(20):1724 ZHAO PP,HUO SX,YAN M,et al.Purification of butin and butein from the Vernonia anthelmintica Willd. and their effects on proliferation and melanogenesis of A375 human melanoma cells[J].China J Chin Mater Med,2013,48(20):1724
[25] HUO SX,WANG Q,LIU XM,et al.The effect of butin on the vitiligo mouse model induced by hydroquinone[J].Phytother Res,2017,31(5):740
[26] DOGRA NK,SURESH K,KRISHANA T,et al.Antipsoriatic effect of fatty acid enriched fraction of Vernonia anthelmintica Willd.fruits[J].J Ethnopharmacol,2018,224:85
[27] LAMBERTINI E,PIVA R,KHAN MT,et al.Effects of extracts from Bangladeshi medicinal plants on in vitro proliferation of human breast cancer cell lines and expression of estrogen receptor alpha gene[J].Int J Oncol,2004,24(2):419
[28] 孙力,尚靖,李红健,等.驱虫斑鸠菊抗肿瘤活性部位的初步研究[J].中国现代中药,2006,8(11):10 SUN L,SHANG J,LI HJ,et al.Studies on anti-tumor activity of extracts of Vernonia anthelmintica Willd.[J].Mod Chin Med,2006,8(11):10
[29] 须玉红,杨荣,金露,等.驱虫斑鸠菊注射液辅助治疗硅沉着病I期兵法肺部感染患者的效果[J].中国医药导报,2015,12(25):133 XU YH,YANG R,JIN L,et al.Effect of Vernonia anthelmintica (L.) Willd injection as an auxiliary treatment for patients with silicosis of stage Ⅰ combined with lung infection[J].China Med Her,2015,12(25):133
[30] LIU GT,LIU YJ,JIN L,et al.Effect of Xinjiang Uyghur,Vernonia anthelmintica Willd. injection treatment with silicosis fibrosis[J].Biomed Res Int,2016:5139651.Published online 2016 Oct 10. doi: 10.1155/2016/5139651
[31] OTARI KV,SHETE RV,UPASANI CD,et al.Evaluation of anti-inflammatory and anti-arthritic activities of ethanolic extract of Vernonia anthelmintica seeds[J].J Cell Tissue Res,2010,10(2):2269
[32] 邓瑞春,周勇,徐建国,等.驱虫斑鸠菊对小鼠免疫分子的影响[J].生物技术通讯,2002,13(3):183 DENG RC,ZOU Y,XU JG,et al.Effects of Vernonia anthelmintica Willd. on the immune fuction in normal mouse in vivo[J].Lett Biotech,2002,13(3):183
[33] SHAIK SF,MADDIRALA DR,KONDETI VK,et al.Antidiabetic and anti-hyperlipidemic activity of ethyl acetate:isopropanol (1:1) fraction of Vernonia anthelmintica seeds in streptozotocin induced diabetic rats[J].Food Chem Toxicol,2010,48(2):495
[34] 刘晓东,闫明,霍仕霞,等.高效液相色谱指纹图谱在驱虫斑鸠菊不同提取工艺对比研究中的应用[J].医学研究生学报,2009,22(8):794 LIU XD,YAN M,HUO SX,et al.High performance liquid chromatography applied to the comparative study of the fingerprints for different extracts of Vernonia anthelmintica (L.) Willd.[J].J Med Postgrad,2009,22(8):794
[35] 赵萍萍,霍仕霞,高莉,等.驱虫斑鸠菊提取物指纹图谱与其对人A375黑素瘤细胞增殖作用的灰关联度分析[J].中国中药杂志,2012,37(5):585 ZHAO PP,HUO SX,GAO L,et al.Grey relational analysis on fingerprint of Vernonia anthelmintica extracts and its effect on proliferation of A375 human melanoma cells[J].China J Chin Mater Med,2012,37(5):585
[36] 刘晓东,闫明,张兰兰,等.驱虫斑鸠菊高效液相色谱指纹图谱分离条件的优化[J].西北药学杂志,2009,24(2):101 LIU XD,YAN M,ZHANG LL,et al.Optimization of separation conditions of fingerprint for Vernonia anthelmintica (L.) Willd. by high performance liquid chromatography[J].Northwest Pharma J,2009,24(2):101
[37] 付瑾,王贝贝,赵爽,等.民族药驱虫斑鸠菊中绿原酸的含量测定[J].中国处方药,2015,13(10):26 FU J,WANG BB,ZHAO S,et al.Determination of chlorogenic acid of Vernonia anthelmintica Willd.[J].J China Prescr Drug,2015,13(10):26
[38] 陈嘉媛,霍仕霞,闫明,等.高效液相色谱法测定驱虫斑鸠菊中异绿原酸A和异绿原酸C含量[J].医药导报,2018,37(11):1393 CHEN JY,HUO SX,YAN M,et al.Determination of isochlorogenic acid A and isochlorogenic acid C in Vernonia anthelmintica by high performance liquid chromatography[J].Her Med,2018,37(11):1393
[39] 范广建,张坚,孙力,等.HPLC法测定驱虫斑鸠菊中紫铆素的含量[J].中国药房,2009,20(30):2366 FAN GJ,ZHANG J,SUN L,et al.Determination of butin in Vernonia anthelmintica by HPLC[J].China Pharm,2009,20(30):2366
[40] 王加利,袁将,全庆华,等.HPLC法同时测定维药驱虫斑鸠菊中4个黄酮类成分含量[J].辽宁中医药大学学报,2017,19(8):45 WANG JL,YUAN J,QUAN QH,et al.Simultaneous determination of four flavonoids in Vernonia anthelmintica Willd.by HPLC[J].J Liaoning Univ Tradit Chin Med,2017,19(8):45
[41] 常虹.新疆地产中药重金属元素含量的测定[J].疾病预防控制通报,2012,27(2):19 CHANG H.Determination of heavy metals in traditional Chinese medicines in Xinjiang[J].Bull Dis Control Prev,2012,27(2):19
[42] 霍仕霞,高莉,彭晓明,等.电感耦合等离子体发射光谱法测定驱虫斑鸠菊中微量元素[J].医药导报,2010,29(2):238 HUO SX,GAO L,PENG XM,et al.Determination of trace elements in Vernonia anthelmintica Willd.by ICP-AES[J].Her Med,2010,29(2):238