⁶⁰Co-γ射线辐照灭菌对六味安消散中指纹图谱和有效成分含量的影响

目的：探索⁶⁰Co-γ射线辐照灭菌对六味安消散有效成分的影响及辐照前后指纹图谱的变化，为六味安消散辐照灭菌提供指导依据。方法：采用Agilent XDB-C₁₈色谱柱（4.6 mm×250 mm，5 μm），以甲醇-0.1%磷酸水溶液为流动相，梯度洗脱，检测波长230 nm；建立六味安消散HPLC指纹图谱，比较0、2、5、10 kGy剂量下⁶⁰Co-γ射线辐照对六味安消散中木香烃内酯、去氢木香烃内酯、芦荟大黄素、大黄素和大黄酚的影响，以及对指纹图谱相似度的影响。结果：当辐照剂量不超过5 kGy时，木香烃内酯、去氢木香烃内酯、大黄素和大黄酚的指纹图谱和含量变化无统计学意义（P>0.05）。结论：⁶⁰Co-γ射线辐照灭菌法对六味安消散有效成分和指纹图谱无影响，可用于六味安消散的灭菌。  

Objective:To investigate the influence of ⁶⁰Co-γ radiation sterilization on the contents of the effective components and the change of fingerprint before and after the radiation sterilization,so as to provide guide for the sterilization of the drug.Methods:The analysis was carried out on an analytical column Agilent XDB-C₁₈(4.6 mm×250 mm,5 μm)with gradient elution of methanol and 0.1% phosphoric acid solution at the detection wavelength of 230 nm. The fingerprint was established.The change of the contents of costunolide,dehydrocostuslactone,emodin and chrysophanol in Liuwei Anxiao powder before and after the ⁶⁰Co-γ radiation sterilization at dose of 0,2,5 and 10 kGy was compared,and the similarities of the fingerprint were also compared.Results:When the radiation dose was less than 5 kGy,the contents and fingerprint of costunolide,dehydrocostuslactone,emodin and chrysophanol had no significant change(P>0.05). Conclusion:⁶⁰Co-γ radiation sterilization has no notable effect on the contents of ostunolide,dehydrocostuslactone,emodin and chrysophanol and fingerprint of Liuwei Anxiao powder,and it can be used for the sterilization of Simiao Junyi powder. 

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