HPLC-DAD法同时测定骨松宝颗粒中5个成分

目的：建立HPLC法同时测定骨松宝颗粒中的5个成分。方法：采用岛津InertSustain C₁₈色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈-0.1%磷酸水溶液，梯度洗脱；体积流量为1.0 mL·min^-1；柱温30℃。结果：芒果苷、芍药苷、阿魏酸、淫羊藿苷、川续断皂苷Ⅵ质量浓度分别在0.512 5~128.1 μg·mL^-1（r=1.000）、4.842~1 211 μg·mL^-1（r=1.000）、0.159 0~39.76 μg·mL^-1（r=1.000）、3.252~812.9 μg·mL^-1（r=1.000）和10.56~2 641 μg·mL^-1（r=1.000）范围内线性关系良好，平均加样回收率（n=6）分别为96.8%（RSD=2.6%）、96.4%（RSD=0.67%）、94.5%（RSD=1.8%）、102.4%（RSD=0.90%）和96.0%（RSD=1.6%）。3批样品中芒果苷、芍药苷、阿魏酸、淫羊藿苷、川续断皂苷Ⅵ的含量分别为0.057 6~0.115 1、0.574 8~0.993 1、0.018 9~0.029 3、0.456 9~1.992 3、1.334 7-3.011 8 mg·g^-1。结论：建立的HPLC方法重复性好，操作简便，可应用于骨松宝颗粒的质量控制。 

Objective:To establish an HPLC method for the simultaneous determination of five components in Gusongbao granules. Methods:The analysis was performed on a thermostatic Shimadzu InertSustain C₁₈ column(250 mm×4.6 mm,5 μm)at 30℃,with the mobile phase comprising of acetonitrile-0.1% phosphoric acid at a flow rate of 1.0 mL·min^-1 in a gradient elution manner. Results:The linear ranges of mangiferin,paeoniflorin, ferulic acid,icariin and asperosaponinⅥ were 0.512 5-128.1 μg·mL^-1(r=1.000),4.842-1 211 μg·mL^-1(r=1.000),0.159 0-39.76 μg·mL^-1(r=1.000),3.252-812.9 μg·mL^-1(r=1.000)and 10.56-2 641 μg·mL^-1,respectively.(r=1.000). The average recoveries were 96.8%(RSD=2.6%),96.4%(RSD=0.67%),94.5%(RSD=1.8%),102.4%(RSD=0.90%)and 96.0%(RSD=1.6%),respectively. The contents of mangiferin,paeoniflorin,ferulic acid,icariin and asperosaponin Ⅵ in three batches of samples were 0.057 6-0.115 1,0.574 8-0.993 1,0.018 9-0.029 3,0.456 9-1.992 3 and 1.334 7-3.011 8 mg·g^-1,respectively. Conclusion:The method is simple and repeatable,which can be used for the quality control of Gusongbao granules. 

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