柴胡桂枝汤高效液相色谱指纹图谱的建立

目的：建立柴胡桂枝汤的HPLC指纹图谱分析方法，为研究柴胡桂枝汤的药效物质基础及质量控制提供科学依据。方法：采用Inertsil ODS-SP C₁₈色谱柱（4.6 mm×150 mm，5 μm），柱温25℃以0.1%甲酸水溶液-0.1%甲酸甲醇溶液为流动相，梯度洗脱，流速0.6 mL·min^-1，检测波长275 nm。采用“中药色谱指纹图谱相似度评价系统2004A版”对12批柴胡桂枝汤HPLC指纹图谱进行评价，并且通过对照品比对及高效液相与质谱联用技术（HPLC-MS）对主要共有峰进行指认。结果：12批柴胡桂枝汤指纹图谱中有21个共有峰，各峰分离度良好，样品间相似度均>0.99，共指认出14个峰。结论：建立的指纹图谱方法具有良好的精密度、重复性、稳定性，各共有峰间分离度高，可为柴胡桂枝汤物质基础和质量控制研究提供参考。

Objective:To establish the chromatographic fingerprint of Chaihu Guizhi decoction by HPLC,in order to provide a scientific basis for the material basis and quality control of Chaihu Guizhi decoction. Methods:The fingerprint was determined by Agilent Inertsil ODS-SP C₁₈ column(4.6 mm×150 mm,5 μm),with the column temperature at 25,and gradient elution with 0.1% formic acid aqueous solution-0.1% formic acid methanol solution at a flow rate of 0.6 mL·min^-1. The detective wavelength was 275 nm. 12 batches of Chaihu Guizhi decoction were evaluated by the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2004A),and the common peaks were identified by comparison with reference substance and HPLC-MS analysis. Results:21 common peaks were found in the fingerprints of 12 batches of Chaihu Guizhi decoction with good separation. The similarities of 12 samples were above 0.99. A total of 14 peaks were identified. Conclusion:The method is precise,reproducible and stable,which can provide reference for material basis and quality control of Chaihu Guizhi decoction.

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