结合拉曼微测技术的TLC-SERS鉴别槲皮素与芦丁

目的：研究槲皮素与芦丁的薄层色谱-表面增强拉曼光谱（TLC-SERS），用于中药中有效成分的鉴别。方法：通过实验考察，确定了激发光波长；采用拉曼微测技术分别扫描涂有表面增强剂的槲皮素与芦丁TLC斑点，获得二维平面分布像图，将激光束快速定位于样品与表面增强剂活位结合点上，得到相应的SERS；并与常规拉曼光谱对比。结果：以780 nm激光为激发波长，银溶胶为表面增强剂，通过比较分析槲皮素与芦丁的TLC-SERS光谱，建立了黄蜀葵花、槐花中有效成分的二维TLC-SERS鉴别法。结论：本方法专属性强，灵敏度高，重复性好，可用于中药的鉴别。

Objective:To study the TLC-SERS of quercetin and rutin for the identification of active ingredients in traditional Chinese medicine. Methods:Through the experimental investigation, the wavelength of the excitation light was selected.The Raman micro-measurement technique was used to scan the spots of quercetin and rutin coated with surface enhancer toobtaina two-dimensional distribution image plane. The corresponding conding point of SERS which was produced by rapid positioning of the laser beam on the sample surface with the enhancer-binding activities was obtained.And then, the results were compared with the ones acquired by regular Raman spectroscopy. Results:The TLC-SERS of quercetin and rutin were established with excitation wavelength at 780 nm, surface enhancer of silver colloids and micro-Raman measurement technology, further successfully applied to identify quercetin in Abelmoschi Corolla and rutin in Sophorae Flos. Conclusion:The method established in this study is found to have significant specificity, high sensitivity and good reproducibility, and can be applied to the identification of traditional Chinese medicine.

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