HPLC-FLD法同时测定不同产地天麻中5个成分含量

目的：建立高效液相色谱-荧光检测方法测定天麻中天麻素、对羟基苯甲醇、巴利森苷A、巴利森苷B、巴利森苷C的含量。方法：采用资生堂MG Ⅲ色谱柱（4.6 mm×250 mm，5 μm），流动相为0.1%甲酸水（A）-乙腈（B），流速为1 mL·min^-1，柱温35℃，进样量5 μL，荧光检测器，激发波长为228 nm，发射波长为300 nm。结果：天麻素、对羟基苯甲醇、巴利森苷A、巴利森苷B、巴利森苷C的线性范围分别在10~5000（r=0.999 6）、10~5 000（r=0.999 9）、250~50 000（r=0.999 6）、250~50 000（r=0.999 9）、250~50 000 ng·mL^-1（r=0.999 7）范围内线性关系良好，平均回收率（n=6）分别为98.5%、97.2%、99.2%、98.7%、96.8%，RSD分别为5.8%、4.6%、0.86%、2.4%、4.8%；28批样品中上述5个成分的含量分别为0.5~20.0、0.1~2.5、0.5~15.4、1.6~8.2、0.7~2.1 mg·g^-1。结论：建立的方法杂质干扰少，分离度好，灵敏度高，线性范围宽，适于天麻成分分析及药材质量控制。

Objective: To establish a high-performance liquid chromatography with fluorescence detector method for the simultaneous determination of five components (gastrodin, 4-hydroxybenzyl alcohol, parishin A, parishin B and parishin C) in Gastrodia Rhizoma. Methods: Chromatographic analysis was conducted on a Shiseido MG Ⅲ column (4.6 mm×250 mm,5 μm),with 0.1% formic acid aqueous solution (A) -acetonitrile (B) as the mobile phase with gradient elution at a flow rate of 1.0 mL·min^-1. The column temperature was 35℃ and the injection volume was 5 μL. The excitation wavelength and emission wavelength for fluorescence detector were 228 nm and 300 nm,respectively. _Result:The linear ranges were within 10-5 000 ng·mL^-1 (r=0.999 6) for gastrodin,10-5 000 ng·mL^-1 (r=0.999 9) for 4-hydroxybenzyl alcohol,250-50 000 ng·mL^-1 (r=0.999 6) for parishin A,250-50 000 ng·mL^-1 (r=0.999 9) for parishin B and 250-50 000 ng·mL^-1 (r=0.999 7) for parishin C. The average recoveries were 97.4%, 96.9%, 99.1%, 98.5% and 96.3% with RSDs of 5.8%, 4.6%, 0.86%, 2.4%and 4.8%, respectively. The contents of gastrodin, 4-hydroxybenzyl alcohol, parishin A, parishin B and parishin C in 28 samples were 0.5-20.0, 0.1-2.5, 0.5-15.4, 1.6-8.2 and 0.7-2.1 mg·g^-1, respectively. Conclusion: The method is sensitive, accurate, and has the good repeatability in separation, which is available for the quality control of Gastrodia Rhizoma.

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