UPLC同时测定野菊花药材中10个黄酮和有机酸的含量

目的：建立UPLC同时测定野菊花药材中绿原酸、咖啡酸、1，3-二咖啡酰奎宁酸、木犀草素-7-O-β-D-葡萄糖苷、3，4-二咖啡酰奎宁酸、3，5-二咖啡酰奎宁酸、4，5-二咖啡酰奎宁酸、木犀草素、蒙花苷及芹菜素10个成分含量的方法。方法：采用Agilent Eclipse Plus C₁₈色谱柱（2.1 mm&#215;100 mm，1.8μm）；以甲醇（A）-0.3%磷酸水溶液（B）为流动相，梯度洗脱，流速0.4 mL&#183;min^-1；检测波长326 nm；柱温25℃。结果：10个成分在38 min内均达到基线分离，线性关系良好（r>0.999 2，n=6）；平均回收率均在96.4%~103.2%（RSD<2.0%，n=6）；测定的6批样品中上述10个成分含量范围分别为1.018~3.430、0.079~0.365、0~0.269、1.198~2.754、0.185~0.765、1.135~6.659、0.587~2.808、0.103~1.663、0.495~13.050、0.214~0.933 mg&#183;g^-1。结论：本方法操作简单，准确性和重复性良好。可用于野菊花药材的多成分同步测定，为野菊花的全面质量评价和质量控制提供科学依据。

Objective: To establish a ultra-performance liquid chromatography (UPLC) method for the simultaneous determination of chlorogenic acid, caffeic acid, 1, 3-dicaffeoylquinic acid, luteolin-7-O-β-D-glucoside, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid, 4, 5-dicaffeoylquinic acid, luteolin, linarin and apigenin in Chrysanthemum indicum. Methods: The separation was carried out on an Agilent Eclipse Plus C₁₈ (2.1 mm&#215;100 mm, 1.8 μm) column eluted with methanol (A) and 0.3% phosphoric acid (B) with gradient mode at a flow rate of 0.4 mL&#183;min^-1. The column temperature was 25℃ and the detection wavelength was set at 326 nm. Results: Good separation of chlorogenic acid, caffeic acid, 1, 3-dicaffeoylquinic acid, luteolin-7-O-β-D-glucoside, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid, 4, 5-dicaffeoylquinic acid, luteolin, linarin and apigenin was achieved within 38 min. Calibration curves of the ten components showed good linear relationship (r>0.999 2, n=6). The average recoveries were within 96.4%-103.2% (RSD<2.0%, n=6). The content ranges of the above mentioned 10 components in 6 batches of samples were 1.018-3.430, 0.079-0.365, 0-0.269, 1.198-2.754, 0.185-0.765, 1.135-6.659, 0.587-2.808, 0.103-1.663, 0.495-13.050 and 0.214-0.933 mg&#183;g^-1, respectively. Conclusion: The method is simple, accurate and repeatable and can be used for simultaneous determination of multi-components in Chrysanthemum indicum, which provides scientific base for comprehensive evaluation and quality control of the herb.

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