HPLC法同时测定抗骨增生丸中毛蕊花糖苷、柚皮苷和淫羊藿苷的含量

目的：建立同时测定抗骨增生丸中毛蕊花糖苷、柚皮苷和淫羊藿苷3种成分含量的高效液相色谱法。方法：应用HPLC-梯度洗脱法，采用C₁₈色谱柱（250 mm×4.6 mm，5 μm），以水为流动相A，甲醇为流动相B，乙腈为流动相C进行梯度洗脱，流速1.0 mL·min^-1，检测波长270 nm，柱温25℃。结果：毛蕊花糖苷、柚皮苷和淫羊藿苷测定浓度的线性范围分别为5.08~101.6、7.56~151.2和5.13~102.6 μg·mL^-1， r均大于0.999；平均加样回收率分别为100.5%、99.5%和99.3%，RSD均小于1.0%。样品中毛蕊花糖苷、柚皮苷、淫羊藿苷含量分别为1.06~1.17、1.75~1.88、1.32~1.53 mg·丸^-1。结论：该实验方法可有效地控制抗骨增生丸的质量。

Objective: To establish an HPLC method for determining three constituents(verbascoside,naringin and icariin)in Kangguzengsheng pills. Methods: The separation was performed on a C₁₈ column(250 mm×4.6 mm, 5 μm)with gradient elution.The mobile phase was composed of water solution(A),methanol(B)and acetonitrile(C)at the flow rate of 1.0 mL·min^-1.The detection wavelength was set at 270 nm and the column temperature was 25℃. Results: The linear ranges were 5.08-101.6 μg·mL^-1 for verbascoside,7.56-151.2 μg·mL^-1 for naringin and 5.13-102.6 μg·mL^-1 for icariin,respectively.The correlation coefficients of all curves were above 0.999.The average recoveries of three analytes were 100.5% for verbascoside,99.5% for naringin,and 99.3% for icariin,with RSD less than 1.0%.The contents of verbascoside,naringin and icariin in 3 samples were 1.06-1.17 mg per pill,1.75-1.88 mg per pill and 1.32-1.53 mg per pill,respectively. Conclusion: The developed HPLC method can be used for the quality control of Kangguzengsheng pills.

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