HPLC法快速测定辅酶Q₁₀维生素E软胶囊中2种功效成分含量

目的：建立HPLC 法同时快速测定辅酶Q₁₀ 维生素E 软胶囊中辅酶Q₁₀ 和维生素E 的含量。方法：以无水乙醇为提取溶剂对样品进行超声提取；选用Accucore C₁₈（150 mm×2.1 mm，2.6 μm）色谱柱，以无水乙醇-甲醇（7：13）为流动相，流速0.4 mL·min^-1，柱温38 ℃，检测波长280 nm。结果：样品中辅酶Q₁₀ 和维生素E 在6 min 内得到很好的分离，质量浓度分别在0.050~0.502 和0.100~0.801 mg·mL^-1 范围内呈良好线性（r=0.999 9）；辅酶Q₁₀ 高、低添加水平的平均回收率（n=3）分别为90.6% 和98.3%，RSD 分别为2.9% 和4.0%；维生素E 高、低添加水平的平均回收率（n=3）为98.9% 和95.7%，RSD 分别为3.1%和2.6%。3 批样品中辅酶Q₁₀ 含量分别为103、102 和105 mg·g^-1，维生素E 含量分别为70.7、71.3 和71.2 mg·g^-1。结论：本文建立的方法经方法学验证，可用于辅酶Q₁₀ 维生素E 软胶囊的质量控制。

Objective: To establish a fast HPLC method for simultaneous determination of coenzyme Q₁₀ and vitamin E in coenzyme Q₁₀ vitamin E softgel. Methods: The sample was extracted by ultrasound with dehydrated alcohol. An Accucore C₁₈ column(150 mm×2.1 mm,2.6 μm)was used as stationary phase and alcohol-methanol (7:13)as the mobile phase. The flow rate was 0.4 mL·min^-1 and the column temperature was set at 38℃. The UV detection wavelength was 280 nm. Results: Coenzyme Q₁₀ and vitamin E were well separated within 6 minutes. The linear ranges of coenzyme Q₁₀ and vitamin E were 0. 050-0. 502 and 0. 100-0. 801 mg·mL^-1(r=0.999 9), respectively. The recoveries(n=3)of coenzyme-Q₁₀ were 90. 6% with RSD of 2. 9% at high concentration and 98.3% with RSD of 4.0% at low concentration;the recoveries(n=3)of vitamin E were 98. 9% with RSD of 3.1% at high concentration and 95.7% with RSD of 2.6% at low concentration. The contents of coenzyme Q₁₀ in the three samples were 103,102 and 105 mg·g^-1,respectively;the contents of vitamin E in the three samples were 70.7,71.3 and 71.2 mg·g^-1,respectively. Conclusion: The proposed method has been validated and it can be used for the quality control of coenzyme Q₁₀ and vitamin E softgel.

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