HPLC法测定阿纳其根中2个N-烷基类成分的含量

目的：建立采用高效液相色谱同时测定维吾尔药阿纳其根中2个N-烷基类成分含量的方法，为制定阿纳其根质量标准提供依据。方法：采用LC-20A型高效液相色谱仪，Agilent TC-C₁₈色谱柱（4.6 mm×150 mm，5 μm），以甲醇-水（70：30）为流动相，流速0.8 mL·min^-1，检测波长243 nm，柱温25℃。结果：十-2E，4E-二烯酸-4-羟基苯乙胺和十二-2E，4E-二烯酸-4-羟基苯乙胺质量浓度分别在30.6~122.4 mg·L^-1和17.2~68.8 mg·L^-1范围内呈良好线性关系，平均加样回收率（n=6）分别为99.90%（RSD=1.60%）和100.4%（RSD=0.85%）；3批阿纳其根药材中两者含量范围分别为0.046%~0.054%和0.035%~0.039%（n=3）。结论：经方法学验证，该方法可为阿纳其根药材质量控制提供一定的参考。

Objective: To establish an HPLC method for the simultaneous determination of dodeca-2E,4E-dienoic acid 4-hydroxyphenylethylamide and deca-2E,4E-dienoic acid 4-hydroxyphenylethylamide in the roots from Anacyclus pyrethrum DC.,whichis a kind of Uyghur medicine.Methods: The analysis was conducted on a type of LC-20A HPLC,and was performed on a Agilent TC-C₁₈(4.6 mm×150 mm,5 μm)column with mobile phase of methanol and water(70:30).The flow rate was 0.8 mL·min^-1,the detection wavelength was 243 nm,and the column temperature was 25℃.The two effective components in 3 batches of medicines were analyzed.Results: The calibration curves of dodeca-2E,4E-dienoic acid 4-hydroxyphenylethylamide and deca-2E,4E-dienoic acid 4-hydroxyphenylethylamide were linear within the ranges of 30.6-122.4 mg·L^-1 and 17.2-68.8 mg·L^-1;the average recovery rates were 99.90% and 100.4% with RSDs of 1.60% and 0.85%(n=6).The content of two components in 3 batches was in a ranges of 0.046%-0.054% and 0.035%-0.039%(n=3),respectively.Conclusion: The method is proved by methodology validation that it can provide some reference for the quality control of Anacyclus pyrethrum DC.

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