HPLC测定疏风散热胶囊中5个化合物的含量

目的：建立高效液相色谱法测定疏风散热胶囊中栀子苷、马钱苷、甘草苷、连翘苷和牛蒡苷的含量。方法：采用Agilent Zorbax SB-Aq C₁₈柱（4.6 mm×250 mm，5 μm），以乙腈-0.2%磷酸水溶液为流动相进行梯度洗脱，流速为1 mL·min^-1，柱温40℃，检测波长为237、228 nm，同时测定疏风散热胶囊中5个化合物的含量。结果：栀子苷、马钱苷、甘草苷、连翘苷和牛蒡苷的线性范围分别为5.500~220.0 μg·mL^-1（r=0.999 9）、1.382~55.30 μg·mL^-1（r=0.999 7）、1.400~56.00 μg·mL^-1（r=0.999 9）、1.377~55.08 μg·mL^-1（r=0.999 8）、20.92~837.0 μg·mL^-1（r=0.999 9）；平均回收率（n=9）分别为97.8%、99.4%、98.1%、101.6%、100.0%。10批疏风散热胶囊中每粒胶囊含上述5个成分的量分别为0.35~1.09、0.03~0.41、0.02~0.13、0.07~0.14、2.38~3.64。结论：建立的方法重复性好，快速，易操作，适用于疏风散热胶囊的质量控制。

Objective: To establish an HPLC method for the simultaneous determination of geniposide,loganin,liquiritin,forsythin and arctiin in Shufengsanre capsules. Methods: Agilent Zorbax SB-Aq C₁₈(4.6 mm×250 mm,5 μm) column was used with acetonitrile-0.2% phosphoric acid solution as mobile phase in gradient elution at a flow rate of 1.0 mL · min^-1,the column temperature was 40℃,and the detection wavelengths were 237 and 228 nm. Results: The linear ranges of geniposide,loganin,liquiritin,forsythin and arctiin were 5.500-220.0 μg·mL^-1(r=0.999 9),1.382-55.30 μg·mL^-1(r=0.999 7)、1.400-56.00 μg·mL^-1(r=0.999 9),1.377-55.08 μg·mL^-1(r=0.999 8) and 20.92-837.0 μg·mL^-1(r=0.999 9),respectively;The average recoveries(n=9) were 97.8%,99.4%,98.1%,101.6% and 100.0%,respectively. The contents for one capsule of geniposide,loganin,liquiritin,forsythin and arctiin in 10 batchs of Shufengsanre capsules were 0.35-1.09,0.03-0.41,0.02-0.13,0.07-0.14 and 2.38-3.64,respectively. Conclusion: The method developed is repeatable,fast and easy and can be used for the quality control of Shufengsanre capsules.