超高效液相色谱法同时测定广藿香中6个成分的含量

目的：建立同时测定广藿香中新西兰牡荆苷2、毛蕊花糖苷、异毛蕊花糖苷、列当苷、藿香黄酮醇和广藿香酮的超高效液相色谱（UPLC）分析方法。方法：采用Waters BEH C₁₈（100 mm×2.1 mm，1.7 μm）色谱柱；流动相为乙腈-0.1%磷酸溶液，梯度洗脱；流速0.4 mL·min^-1；柱温30℃；检测波长309 nm。结果：新西兰牡荆苷2、毛蕊花糖苷、异毛蕊花糖苷、列当苷、藿香黄酮醇、广藿香酮的线性范围分别为2.18~109.00、8.66~433.11、2.12~106.00、1.81~90.40、0.90~45.00、1.09~54.24 μg·mL^-1（r²>0.999 7）；平均回收率（n=9）为99.1%~100.7%，精密度、重复性、稳定性均符合有关规定；10批样品中6个成分的含量范围分别为0.137~0.959、0.980~5.877、0.306~1.363、0.273~1.169、0.136~0.387、0.292~1.619 mg·g^-1。结论：本法经方法学验证，可用于广藿香质量控制。

Objective:To establish a UPLC method for simultaneous determination of vicenin-2,verbascoside,isoverbascoside,crenatoside,pachypodol and pogostone in Pogostemonis Herba. Methods:Analysis was performed on a Waters BEH C₁₈ column(100 mm×2.1 mm,1.7μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid in gradient mode. The flow rate was 0.4 mL·min^-1. The column temperature was maintained at 30℃ and the detection wavelength was set at 309 nm. Results:Vicenin-2,verbascoside,isoverbascoside,crenatoside,pachypodol and pogostone showed a good linear relationship within the ranges of 2.18-109.00 μg·mL^-1,8.66-433.11 μg·mL^-1,2.12-106.00 μg·mL^-1,1.81-90.40 μg·mL^-1,0.90-45.00 μg·mL^-1 and 1.09-54.24 μg·mL^-1,respectively(r²>0.999 7). The average recoveries of the six compounds were within 99.1%-100.7%. Results of precision,stability and reproducibility test were satisfactory. The contents of six components in ten batches of samples were 0.137-0.959 mg·g^-1,0.980-5.877 mg·g^-1,0.306-1.363 mg·g^-1,0.273-1.169 mg·g^-1,0.136-0.387 mg·g^-1 and 0.292-1.619 mg·g^-1,respectively. Conclusion:The established method can be used for the quality control of Pogostemonis Herba.