目的：建立多指标含量测定结合化学计量学分析法，评价加味逍遥丸质量。方法：样品经甲醇超声提取，采用Kromasil C18色谱柱（250 mm×4.6 mm，5 μm），以乙腈-0.2%磷酸水溶液进行梯度洗脱，234 nm和440 nm双波长检测，并使用雷达图分析、聚类热图分析和夹角余弦相似度分析方法对数据进行统计。结果：样品中芍药苷、丹皮酚、阿魏酸、甘草苷、甘草酸、栀子苷、西红花苷Ⅰ和西红花苷Ⅱ的含量分别为1.484~5.508、0.94~3.28、0.014~0.147、0.23~1.79、0.353~2.488、2.40~11.33、0.008 6~0.731 1和0.001 6~0.072 3 mg·g-1。化学计量学分析表明各厂家的样品间存在一定差异，同一厂家的样品较为一致。区分各类样品的强特征峰为栀子苷峰、芍药苷峰、丹皮酚峰、栀子peak 6、甘草苷峰、西红花苷Ⅰ峰和牡丹皮peak 2。结论：该法准确、专属，适用于加味逍遥丸整体质量评价。
Objective:To establish a method for overall quality evaluation of Jiawei Xiaoyao pills by multi-component determination and chemometric analysis.Methods:The samples were extracted by ultrasonic with methanol.Separation was performed on a Kromasil ODS column(250 mm×4.6 mm,5 μm)with a gradient elution of acetonitrile and 0.2% phosphoric acid.The detection wavelength was at 234 nm and 440 nm.The radar chart,HCA heatmap and cosine similarity were used for data analysis.Results:The content of paeoniflorin,paeonol,ferulic acid,liquiritin,glycyrrhizic acid,geniposide,crocin Ⅰand crocin Ⅱ were 1.484-5.508 mg·g-1,0.94-3.28 mg·g-1,0.014-0.147 mg·g-1,0.23-1.79 mg·g-1,0.353-2.488 mg·g-1,2.40-11.33 mg·g-1,0.008 6-0.731 1 mg·g-1 and 0.001 6-0.072 3 mg·g-1,respectively.Chemometric analysis showed that there were some differences among the samples from different manufacturers,and the samples from the same manufacturer were more consistent.Strong characteristic peaks for discrimination of different samples were geniposide peak,paeoniflorin peak,paeonol peak,peak 6 of Gardeniae Fructus,liquiritin peak,crocin Ⅰ peak and peak 2 of Moutan Cortex.Conclusion:The method is accurate and specific,which can be used for overall quality evaluation of Jiawei Xiaoyao pills.
