基于SERS技术检测牛奶中环丙沙星的研究

目的：建立牛奶中环丙沙星的表面增强拉曼光谱（SERS）检测方法。方法：对环丙沙星的主要拉曼峰进行归属，选取1 384 cm^-1拉曼特征峰，在最佳实验条件下进行了SERS检测。结果：环丙沙星在牛奶中检测下限约为50 μg·mL^-1，牛奶溶液中特征峰强度与浓度的曲线方程为Y=224.244 8+2.582 4X，R²=0.939，回收率在92.7%~108.3%之间，RSD小于8.3%。结论：该方法具有快速、准确、无损及操作简便等优点，在牛奶中环丙沙星的检测具有一定的应用潜力。

Objective:To establish a method for the surface-enhanced Raman spectroscopy (SERS)detection of ciprofloxacin in milk.Methods:Main Raman vibration characteristic peaks of the ciprofloxacin molecules were identified.The Raman characteristic peak at 1 384 cm^-1 was selected and the SERS detection of ciprofloxacin was carried out under the optimal experimental conditions.Results:The limit of detection of ciprofloxacin was 50 μg·mL^-1 in milk.The equation curve of the characteristic peak intensity and concentrations in milk was Y=224.244 8+2.582 4X.The linear correlation coefficient (R²)was 0.939.The recovery of this method ranges from 92.7% to 108.3%.The RSD is less than 8.3%.Conclusion:The SERS detection method is rapid,accurate,nondestructive and easy to operate.It has a good potential in terms of ciprofloxacin detection in milk.