HPLC法同时测定咳速停糖浆中5个生物碱的含量

目的：建立同时、简便测定咳速停糖浆中5个生物碱（吗啡、盐酸麻黄碱、盐酸伪麻黄碱、磷酸可待因、盐酸罂粟碱）含量的HPLC分析方法。方法：采用Welch Ultimate^®AQ-C₁₈色谱柱（4.6 mm×250 mm，5 μm），以乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱，流速1.0 mL·min^-1，检测波长为210 nm（吗啡、麻黄碱、伪麻黄碱、可待因）与250 nm（罂粟碱），柱温35℃。结果：5个生物碱类成分质量浓度分别在0.9~7.48 μg·mL^-1（r=0.999 1）、2.43~20.24 μg·mL^-1（r=0.999 9）、4.49~37.44 μg·mL^-1（r=0.999 2）、0.43~3.58 μg·mL^-1（r=0.999 5）、0.09~0.79 μg·mL^-1（r=0.999 8）范围内线性关系良好，平均回收率（n=9）分别为99.1%、102.5%、107.5%、100.1%、94.0%，相应的RSD分别为3.7%、3.4%、2.3%、1.5%、1.2%。10批咳速停糖浆样品中上述5个生物碱的含量范围分别为8.46~21.71、28.18~153.9、26.58~196.9、4.23~15.30、1.20~2.89 μg·mL^-1。结论：该方法为咳速停糖浆质量标准的提升提供参考依据。

Objective: To establish a method for simultaneous and convenient determination of five kinds of alkaloids(morphine,ehpedrine hydrochloride,pseudophedrine hydrochloride,codeine phosphate,papaverine hydrochloride)in Kesuting syrup by HPLC analytical method.Methods: Welch Ultimate^® AQ-C₁₈ column(4.6 mm×250 mm,5 μm)was adopted,the mobile phase was acetonitrile and 0.1% phosphoric acid with gradient elution at a flow rate of 1.0 mL·min^-1.The detection wavelength was 210 nm for morphine,ehpedrine, pseudophedrine and codeine,and 250 nm for papaverine.The column temperature was 35℃.Results: Good linear relationship was observed for morphine,ehpedrine hydrochloride,pseudophedrine hydrochloride,codeine phosphate and papaverine hydrochloride in the range of 0.9-7.48 μg·mL^-1(r=0.999 1),2.43-20.24 μg·mL^-1(r=0.999 9),4.49-37.44 μg·mL^-1(r=0.999 2),0.43-3.58 μg·mL^-1(r=0.999 5)and 0.09-0.79 μg·mL^-1(r=0.999 8),respectively.The average recoveries(n=9)were 99.1%(RSD=3.7%),102.5%(RSD=3.4%),107.5%(RSD=2.3%),100.1%(RSD=1.5%)and 94.0%(RSD=1.2%),respectively.The content ranges of the above-mentioned five alkaloids were 8.46-21.71 μg·mL^-1,28.18-153.9 μg·mL^-1,26.58-196.9 μg·mL^-1,4.23-15.30 μg·mL^-1 and 1.20-2.89 μg·mL^-1 respectively in 10 Kesuting syrup.Conclusion: This method provided reference basis for the improvement of the quality standard of Kesuting syrup.