HPLC法同时测定连钱草中4个酚酸类成分的含量

目的：建立同时测定连钱草中咖啡酸、咖啡酰羟基乙酸、迷迭香酸和丹酚酸A 4个酚酸类成分含量的高效液相色谱法。方法：采用CAPCELL PAK C₁₈色谱柱（250 mm&#215;4.6 mm，5 μm），以乙腈（A）-0.1%甲酸水溶液（B）为流动相，梯度洗脱，流速1.0 mL&#183;min^-1，检测波长330 nm，柱温30℃。结果：咖啡酸、咖啡酰羟基乙酸、迷迭香酸和丹酚酸A进样量分别在0.034~0.85 μg（r=0.999 8）、0.014~0.35 μg（r=0.999 8）、0.524 5~13.112 5 μg（r=0.999 8）和0.008 4~0.21 μg（r=0.999 9）范围内与峰面积呈现良好的线性关系；平均回收率（n=3）分别为98.2%~99.6%（RSD<0.8）、97.6%~99.4%（RSD<1.3%）、100.2%~100.5%（RSD<1.0%）和98.1%~99.2%（RSD<1.1%）；8批样品中咖啡酸、咖啡酰羟基乙酸、迷迭香酸和丹酚酸A含量范围分别为0.238~1.121、0.036~0.180、1.461~11.011和0.042~0.124 mg&#183;g^-1。结论：该方法可用于连钱草的质量控制。

Objective: To establish an HPLC method for the simultaneous determination of four phenolic acids in Herba Glechomae including caffeic acid,caffeoylglycolic acid,rosmarinic acid and salvianolic acid A.Methods: The CAPCELL PAK C₁₈ column(250 mm&#215;4.6 mm,5 μm)was used for separation.And the mobile phase consisted of acetonitrile(A)-0.1% formic acid(B)with gradient elution at a flow rate of 1.0 mL&#183;min^-1.The detection wavelength was 330 nm and the column temperature was at 30℃.Results: The linear range of caffeic acid,caffeoylglycolic acid,rosmarinic acid and salvianolic acid A were 0.034-0.85 μg (r=0.999 8),0.014-0.35 μg(r=0.999 8),0.524 5-13.112 5 μg(r=0.999 8)and 0.008 4-0.21 μg(r=0.999 9),respectively.The average recoveries(n=3)were 98.2%-99.6%(RSD<0.8%),97.6%-99.4%(RSD<1.3%),100.2%-100.5%(RSD<1.0%)and 98.1%-99.2%(RSD<1.1%),respectively.The contents of caffeic acid,caffeoylglycolic acid,rosmarinic acid and salvianolic acid A in eight batches of samples were 0.238-1.121,0.036-0.180,1.461-11.011 and 0.042-0.124 mg&#183;g^-1,respectively.Conclusion: The method can be used to control the quality of the Herba Glechomae.