RP-HPLC测定叶黄素脂质体的含量

目的：建立反相高效液相色谱法（RP-HPLC）测定叶黄素脂质体的含量。方法：采用Diamonsil^（2） C₁₈（5 μm，250 mm×4.6 mm）色谱柱，以100%纯乙腈为流动相，流速0.6 mL·min^-1，检测波长为443 nm，进样量20 μL。结果：叶黄素的线性范围为6.96~69.57 μg·mL^-1（r=0.999 8），平均加样回收率（n=9）为98.8%。3批样品的含量分别为98.61%、96.17%、100.51%。结论：本方法适用于叶黄素脂质体的含量测定。

Objective: To establish an RP-HPLC method for the determination of lutein liposomes.Methods: A Diamonsil⁽²⁾ C₁₈ column(5 μm,250 mm×4.6 mm) was adopted as the analytical column.The mobile phase consisted of 100% acetonitrile with isocratic elution at a flow rate of 0.6 mL·min^-1;the detection wavelength was 443 nm and the injection volume was 20 μL.Results: The linear range was 6.96-69.57 μg·mL^-1(r=0.999 8) for lutein and the average recovery(n=9) was 98.8%.The content in three batches of samples was 98.61%,96.17% and 100.51%,respectively.Conclusion: The method can be used for determining the content of lutein liposomes.

[1] MA L,LIN XM.Effects of lutein and zeaxanthin on aspects of eye health[J].J Sci Food Agric,2010,90(1):2
[2] KIJLSTRA A,TIAN Y,KELLY ER,et al.Lutein:More than just a filter for blue light[J].Prog Retin Eye Res,2012,31(4):303
[3] GUNASEKERA RS,SEWGOBIND K,DESAI S,et al.Lycopene and lutein inhibit proliferation in rat prostate carcinoma cells[J].Nutr Cancer,2007,58(2):171
[4] RICCIONI G,SPERANZA L,PESCE M,et al.Novel phytonutrient contributors to antioxidant protection against cardiovascular disease[J].Nutrition,2012,28(6):605
[5] ROBERTS RL.Lutein,zeaxanthin,and skin health[J].Am J Lifest Med,2013,7(3):182
[6] CHEW EY,CLEMONS TE,SANGIOVANNI JP,et al.Secondary analyses of the effects of lutein/zeaxanthin on age-related macular degeneration progression:AREDS2 report No.3[J].Jama Ophthalmol,2014,132(2):142
[7] 焦岩,李大婧,刘春泉,等.叶黄素纳米脂质体的制备工艺优化及其氧化稳定性[J].食品科学,2017,38(18):259 JIAO L,LI DJ,LIU CQ,et al.Preparation condition optimization of lutein nanoliposomes and oxidative stability[J].Food Sci,2017,38(18):259
[8] Al-JAMAL WT,KOSTA RELOS K.Liposomes:from a clinically established drug delivery system to a nanoparticle platform for theranostic nanomedicine[J].Acc Chem Res,2011,44(10):1094
[9] TAN C,XIS S,XUE J,et al.Liposomes as vehicles for lutein:preparation,stability,liposomal membrane dynamic and structure[J].J Agric Food Chem,2013,61(34):8175
[10] 林宏琳,钟碧萍,林国斌.对照品替代法用于缓解视疲劳片叶黄素含量测定的研究[J].药物分析杂志,2014,34(5):934 LIN HL,ZHONG BP,LIN GB.Quantitative determination of lutein in visual fatigue relieving tablets by reference substance substitution[J].Chin J Pharm Anal,2014,34(5):934
[11] 李宁,张雪霞,崔彦,等.HPLC同时测定万寿菊花中叶黄素及玉米黄素的含量[J].中国新药杂志,2006,15(16):1381 LI N,ZHANG XX,CUI Y,et al.Determination of lutein and zeaxanthin in Tagetes erecta L.by HPLC[J].Chin New Drugs J,2006,15(16):1381
[12] JIANG XY,CHEN LS,ZHOU CS.Lutein and lutein esters in marigold flowers by high performance liquid chromatography[J].J Cent South Univ,2005,12(3):306
[13] 孙丽丽,张智杰,郑永杰.玉米黄色素的提取和分析[J].齐齐哈尔大学学报(自然科学版),2009,25(1):74 SUN LL,ZHANG ZJ,ZHENG YJ.Extraction and analysis of maize yellow pigment[J].J Qiqihar Univ(Nat Sci Ed),2009,25(1):74