双波长-UPLC法同时测定知柏地黄丸中6个成分的含量

目的：建立双波长-UPLC法同时测定知柏地黄丸中莫诺苷、芒果苷、马钱苷、芍药苷、小檗碱和丹皮酚6个成分含量。方法：采用C₁₈色谱柱（2.1 mm×100 mm，1.7 μm），流动相为乙腈-0.1%磷酸水溶液，梯度洗脱，流速0.4 mL·min^-1，检测波长236 nm（莫诺苷、芒果苷、马钱苷、芍药苷）、274 nm（小檗碱、丹皮酚），柱温40℃。结果：仅需12 min即可完成测试，莫诺苷、芒果苷、马钱苷、芍药苷、小檗碱、丹皮酚的线性范围分别为2.488~124.4、2.506~125.3、4.404~220.2、2.672~133.6、5.870~293.5和4.795~239.8 ng，r不小于0.999 8；加样回收率（n=9）分别为100.0%、101.3%、100.8%、101.6%、100.5%、98.4%；20批样品中上述成分的含量测定结果分别为0.37~1.04、0.05~0.41、0.50~0.75、0.43~0.83、0.57~1.02、0.77~1.33 mg·g^-1。结论：建立的方法可用于知柏地黄丸的质量控制。

Objective:To develop a method for simultaneous determination of morroniside,mangiferin,loganin,peoniflorin,berberine and paeonol in Zhibai Dihuang pills by dual-wavelength UPLC.Methods:The separation was performed on a C₁₈ (2.1 mm×100 mm,1.7 μm) and the column temperature was set at 40℃.The mobile phase was composed of acetonitrile and 0.1% phosphoric acid with gradient elution at a flow rate of 0.4 mL·min^-1.The detection wavelength was set at 236 nm for morroniside,mangiferin,loganin and peoniflorin and at 274 nm for berberine and paeonol.Results:The analysis time was only 12 min.The linear ranges of morroniside,mangiferin,loganin, peoniflorin,berberine and paeonol were 2.488-124.4 ng,2.506-125.3 ng,4.404-220.2 ng,2.672-133.6 ng,5.870-293.5 ng and 4.795-239.8 ng (r ≥ 0.999 8),respectively.The recoveries (n=9) of the six compounds were 100.0%,101.3%,100.8%,101.6%,100.5% and 98.4%,respectively.The contents of above-mentioned six components in 20 samples were 0.37-1.04 mg·g^-1,0.05-0.41 mg·g^-1,0.50-0.75 mg·g^-1,0.43-0.83 mg·g^-1,0.57-1.02 mg·g^-1 and 0.77-1.33 mg·g^-1,respectively.Conclusion:The method can be used for quality control of Zhibai Dihuang pills.