HPLC-ELSD同时测定不同采收期的细梗香草中2种皂苷的含量

目的：通过测定并比较不同采收期细梗香草中皂苷B和皂苷C的含量，确定细梗香草最佳采收期。方法：采用Platisil C₁₈色谱柱（250 mm×4.6 mm，5 μm），以乙腈-0.3%醋酸水（47：53）为流动相，流速1.0 mL·min^-1，漂移管温度90℃，载气流速1.0 L·min^-1。结果：细梗香草皂苷B和C进样量分别在0.20~10.08 μg和0.24~6.10 μg范围内线性关系良好（r=0.999 7）；平均加样回收率（n=9）分别为96.1%、96.7%，RSD（n=9）分别为0.99%、1.2%。不同采收期细梗香草中皂苷B和皂苷C含量发生显著变化，2种皂苷类成分在7月和11月的含量相对最高。结论：本法可同时测定细梗香草中细梗香草皂苷B和C的含量。皂苷B和皂苷C含量随采收期不同而异，其总量最大值出现在7月和11月。

Objective:To determine and compare contents of capiiliposides B and C in Lysimachia capillipes in different harvest time,so as to optimize the best harvest time.Methods:The Platisil C₁₈ column (250 mm×4.6 mm,5 μm) was used.Acetonitrile-0.3% phosphoric acid (47:53) was used as the mobile phase with isocratic elution at a flow rate of 1.0 mL·min^-1.The tube temperature of ELSD was 90℃ and the flow rate of carrier gas was 1.0 L·min^-1.Results:The calibration curves of capiiliposides B and C were in good linearity over 0.20-10.08μg and 0.24-6.10 μg(r=0.999 7),respectively.The average recoveries were 96.1% and 96.7% with RSD of 0.99% and 1.2%.Contents of two saponins in Lysimachia capillipes in different harvest time varied a lot and the higher contents were observed in samples harvested in July and November.Conclusion:The established method could determine capiiliposides B and C in Lysimachia capillipes simultaneously.The contents of the two components varied by different harvest time.The maximum total contents were observed in July and November.