目的：建立UFLC-QTRAP-MS/MS的方法，同时测定不同产地绞股蓝及其商品药材中10个化合物（胸苷、尿苷、腺苷、肌苷、2'-脱氧鸟苷、鸟苷、腺嘌呤、次黄嘌呤、尿嘧啶、鸟嘌呤）的含量。方法：采用XBridge Amide色谱柱（2.1 mm×100 mm，3.5 μm），以0.2%甲酸水溶液（A）-0.2%甲酸乙腈溶液（B）为流动相，0.6 mL·min-1流速梯度洗脱，正离子（ESI+）模式下多反应监测（MRM）检测。结果：尿嘧啶、胸苷、尿苷、次黄嘌呤、肌苷、2'-脱氧鸟苷、腺苷、鸟苷、腺嘌呤、鸟嘌呤的线性范围分别为1.97~3 936.00、2.00~200.00、30.48~3 048.00、19.84~1 984.00、10.16~1 016.00、4.95~990.00、19.72~1 972.00、14.70~2 940.00、1.96~196.00、14.76~1 476.00 ng·mL-1（r > 0.997 3），平均加样回收率为99.3%~101.3%，含量分别为181.54~1 604.95、0.00~216.45、63.65~1 093.17、3.48~555.12、0.07~215.66、0.00~171.65、0.00~338.63、24.67~966.08、0.37~140.07、14.89~830.79 μg·g-1，绞股蓝中以尿嘧啶、尿苷、鸟苷、鸟嘌呤含量较高；不同产地及商品药材中10个成分含量有所差异。结论：该方法为绞股蓝药材内在质量的综合评价提供新的科学依据。
Objective: To establish a method for simultaneous determination of 10 components(thymidine,uridine,adenosine,inosine,2'-deoxyguanosine,guanosine,adenine,hypoxanthine,uracil and guanine) in Gynostemma pentaphyllum from different habitats and commercial sources by ultra-fast liquid chromatography tandem combined with triple quadrupole mass spectrometry(UFLC-QTRAP-MS/MS).Methods: The analysis was carried out on an XBridge Amide column(2.1 mm×100 mm,3.5 μm) which was eluted by a mobile phase of 0.2% formic acid-0.2% formic acid in acetonitrile at a flow rate of 0.6 mL·min-1.The target compounds were analyzed by the positive ion multiple reaction monitoring(MRM) mode.Results: Uracil,thymidine,uridine,hypoxanthine,inosine, 2'-deoxyguanosine,adenosine,guanosine,adenine and guanine showed good linearity(r > 0.997 3) in the range of 1.97-3 936.00 ng·mL-1,2.00-200.00 ng·mL-1,30.48-3 048.00 ng·mL-1,19.84-1 984.00 ng·mL-1,10.16-1 016.00 ng·mL-1,4.95-990.00 ng·mL-1,19.72-1 972.00 ng·mL-1,14.70-2 940.00 ng·mL-1,1.96-196.00 ng·mL-1 and 14.76-1 476.00 ng·mL-1,respectively.The average recoveries of the ten components were between 99.3% and 101.3%.The contents of ten components were in the range of 181.54-1 604.95μg·g-1,0.00-216.45 μg·g-1,63.65-1 093.17 μg·g-1,3.48-555.12 μg·g-1,0.07-215.66 μg·g-1,0.00-171.6 5 μg·g-1,0.00-338.63 μg·g-1,24.67-966.08 μg·g-1,0.37-140.07 μg·g-1 and 14.89-830.79 μg·g-1,respectively.The contents of uracil,uridine,guanosine and guanine were higher in Gynostemma pentaphyllum.The contents of ten components varied by habitats and commercial sources.Conclusion: The established method provides scientific basis for the comprehensive quality evaluation of Gynostemma pentaphyllum.
