HPLC同时测定鸡血藤中芒柄花素和美迪紫檀素等6个黄酮类成分

目的：建立HPLC同时测定鸡血藤中原儿茶酸、儿茶素、表儿茶素、甘草素、芒柄花素、美迪紫檀素含量的方法，为鸡血藤药材质量标准改进提供参考。方法：采用ZORBAX SB-C₁₈色谱柱（4.6 mm×150 mm，5 μm），以乙腈（A）-0.2%磷酸（B）为流动相进行梯度洗脱，流速1 mL·min^-1，检测波长为260 nm（检测原儿茶酸、芒柄花素）和280 nm（检测儿茶素、表儿茶素、甘草素、美迪紫檀素），柱温30℃。结果：原儿茶酸、儿茶素、表儿茶素、甘草素、芒柄花素、美迪紫檀素的质量浓度分别在2.286~228.6 μg·mL^-1（r=0.999 9）、2.174~217.4 μg·mL^-1（r=0.999 9）、2.79~279 μg·mL^-1（r=0.999 9）、0.424~42.4 μg·mL^-1（r=0.999 9）、2.426~242.6 μg·mL^-1（r=0.999 9）、0.339~33.92 μg·mL^-1（r=0.999 8）的范围内线性关系良好，加样回收率分别为99.8%（RSD=1.0%）、99.8%（RSD=2.5%）、101.2%（RSD=2.3%）、100.8%（RSD=1.8%）、100.9%（RSD=2.3%）、100.6%（RSD=2.4%）。11批药材中原儿茶酸、儿茶素、表儿茶素、甘草素、芒柄花素、美迪紫檀素含量范围分别为0.250~0.898、1.447~5.662、1.759~11.925、0.035~0.134、0.218~0.535、0.065~0.585 mg·g^-1。结论：该方法可用于同时测定鸡血藤中6个黄酮类成分的含量，为该药材的质量标准改进提供参考。

Objective: To establish an HPLC method for simultaneous determination of protocatechuic acid,catechol,L-epicatechin,liquiritigenin,formononetin and medicarpinin Spatholobi Caulis,and to offer reference for improvement of the standard of the drug.Methods: Separation was performed on a ZORBAX SB-C₁₈ column(4.6 mm×150 mm, 5 μm) and the mobile phase was acetonitrile-0.2% phosphoric acid with gradient elution.The flow rate was 1 mL·min^-1 and the wavelength was set at 260 nm for protocatechuic acid and formononetin and at 280 nm for catechol,L-epicatechin,liquiritigenin and medicarpin.The column temperature was 30.Results: The linear ranges of protocatechuic acid,catechol,L-epicatechin,liquiritigenin,formononetin and medicarpin were 2.286-228.6 μg·mL^-1(r=0.999 9),2.174-217.4 μg·mL^-1(r=0.999 9),2.79-279μg·mL^-1(r=0.999 9),0.424-42.4 μg·mL^-1(r=0.999 9),2.426-242.6 μg·mL^-1(r=0.999 9) and 0.339-33.92 μg·mL^-1(r=0.999 8), respectively.The average recoveries(n=6) were 99.8%(RSD=1.0%),99.8%(RSD=2.5%),101.2%(RSD=2.3%),100.8%(RSD=1.8%),100.9%(RSD=2.3%) and 100.6%(RSD=2.4%),respectively.The content ranges of protocatechuic acid,catechol,L-epicatechin,liquiritigenin,formononetin and medicarpinin Spatholobi Caulis were 0.250-0.898 mg·g^-1,1.447-5.662 mg·g^-1,1.759-11.925 mg·g^-1,0.035-0.134 mg·g^-1,0.218-0.535 mg·g^-1 and 0.065-0.585 mg·g^-1,respectively.Conclusion: The developed method is suitable for simultaneous determination of 6 components in Spatholobi Caulis,thus providing reference for the improvement of the standard of the drug.