保健食品中一个新型他达拉非类似物的分析鉴定

目的：鉴定保健食品中非法添加的一个未知结构的他达拉非类似物。方法：采用高效液相色谱-二极管阵列检测器联用（HPLC-DAD）技术进行补肾壮阳类保健食品非法添加筛查时发现一个未知的他达拉非类似物，经过正相硅胶薄层色谱分离纯化得到目标化合物后，用超高效液相色谱-二级质谱联用（UPLC-MS/MS）技术获得其分子量和结构碎片，用核磁共振得到碳谱和氢谱数据，结合文献分析，最终鉴定该化合物的结构。结果：在保健食品中发现了一个新型他达拉非类似物-二乙氨基前他达拉非。结论：该化合物不在现有补肾壮阳类中成药检验标准11种目标化合物范围内，是一种新的非法添加化学物质。

Objective: To identify an unknown tadalafil analogue illegally adulterated in health foods. Methods: An unknown tadalafil analogue was observed in a routine screening for compounds illegally adulterated in heath foods by HPLC-DAD. The target compound was isolated by silica gel thin layer chromatography. Its molecular weight and structural fragments were obtained by UPLC-MS/MS, and its ¹³C-NMR and ¹H-NMR spectum were also obtained using nuclear magnetic resonance. Its structure was identified by analysis of these data in combination with literature analysis. Results: A new tadalafil analogue, diethylaminopretadalafil, was detected in health foods. Conclusion: It is a new compound illegally adulterated in health foods. The current inspection standard, which lists 11 target compounds, does not include this compound.

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