目的：建立UPLC-MS 法同时测定补中益气丸中甘草苷、柚皮苷、橙皮苷、新橙皮苷、甘草素、橙皮素、柚皮素、升麻素、黄芪甲苷、人参皂苷 Rb1、人参皂苷 Rb2、人参皂苷 Rd、人参皂苷 Rg1、甘草酸和柴胡皂苷 a 的含量。方法：采用Zorbax Eclipse Plus C18色谱柱（100 mm×2.1 mm，1.8 μm）；流动相为0.1%甲酸乙腈溶液-0.1%甲酸溶液，梯度洗脱；质谱离子化方式为负源电喷雾（ESI-），定量分析采用多反应离子检测模式（MRM）。结果：各化合物在相应质量浓度范围内呈良好的线性关系，r2值为0.992~0.998；15种化合物低高浓度下的精密度RSD 分别在1.2%~4.56%和1.6%~7.4%之间； 15种化合物的平均加样回收率为95.8%~104.3%，相应的RSD在2.4%~8.7%间；实际样品中15种成分的平均含量在0.187~3.076 μg·丸-1。结论：分析一步完成，降低了分析成本，因此该方法简便、高灵敏度、高通量，是检测补中益气丸中药制剂的可靠方法。
Objective: To establish a UPLC-MS method for simultaneous determination of liquiritin, naringin, hesperidin, neohesperidin, liquiritigenin, hesperetin, naringenin, cimifugin, astragalosideIV, ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rd, ginsenoside Rg1, glycyrrhizic acid, saikosaponin in Buzhong Yiqi pills.Methods: Agilent Zorbax Eclipse Plus C18(100 mm×2.1 mm, 1.8 μm)column was used. The gradient mobile phase consisted of water and acetonitrile both containing 0.1% formic acid. Mass spectrometer was operated using electrospray ionization(ESI-)source in the negative ion mode for detection. Mass data acquisition was done with multiple reaction monitoring(MRM). Results: Full validation of the assay was implemented. Excellent linear calibration curves were obtained over the tested concentration ranges(r2, 0.992-0.998). Intra-and inter-day precisions(RSD)of low and high quality controls were 1.2%-4.6% and 1.6%-7.4%, respectively. The mean extraction recoveries were all between 95.8% and 104.3%, and the RSD values were 2.4%-8.7%. In the analysis of commercial samples, the mean contents of the tested analytes were 0.187-3.076 μg per pill. Conclusion: This method significantly reduces the analysis cost. This new, reliable and high throughput method is beneficial for quantification assays of multiply components in Buzhong Yiqi pills.
