HPLC法同时测定排石颗粒中3个酚酸类成分的含量

目的：建立同时测定排石颗粒中咖啡酰甘醇酸、迷迭香酸和丹酚酸A 3个酚酸类成分含量的高效液相色谱法。方法：采用SunFire C₁₈色谱柱（250 mm×4.6 mm，5 μm），以乙腈（A）-0.1%甲酸水溶液（B）为流动相，梯度洗脱，流速1.0 mL·min^-1，检测波长330 nm，柱温30℃。结果：咖啡酰甘醇酸、迷迭香酸和丹酚酸A进样量分别在0.026 8~0.535 0 μg（r=0.999 9）、0.261 8~5.236 0 μg（r=0.999 9）和0.010 2~0.203 6μg（r=0.999 9）范围内与峰面积呈现良好的线性关系；平均回收率（n=6）分别为95.6%、99.0%和97.6%，RSD分别为1.1%、1.1%和1.4%；13批样品中咖啡酰甘醇酸、迷迭香酸和丹酚酸A含量范围分别为15.06~25.33、118.24~264.13和8.78~18.30 μg·g^-1。结论：该方法可用于排石颗粒的质量控制。

Objective:To establish an HPLC method for the simultaneous determination of three phenolic acids in Paishi granules,namely,caffeoylglycolic acid,rosmarinic acid and salvianolic acid A.Methods:The SunFire C₁₈ column(250 mm×4.6 mm,5 μm)was used,the mobile phase was acetonitrile(A)-0.1% phosphoric acid(B)with gradient elution at a flow rate of 1.0 mL·min^-1.The detection wavelength was 330 nm and the column temperature was at 30℃.Results:The linear ranges of caffeoylglycolic acid,rosmarinic acid and salvianolic acid A were 0.026 8-0.535 0 μg(r=0.999 9),0.261 8-5.236 0 μg(r=0.999 9)and 0.010 2-0.203 6 μg(r=0.999 9), respectively.The average recoveries(n=6)were 95.6%(RSD=1.1%),99.0%(RSD=1.1%)and 97.6%(RSD=1.4%)respectively.The contents of caffeoylglycolic acid,rosmarinic acid and salvianolic acid A in thirteen batches of samples were 15.06-25.33 μg·g^-1,118.24-264.13 μg·g^-1 and 8.78-18.30 μg·g^-1,respectively.Conclusion:The method can be used to control the quality of Paishi granules.