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1α,25-(OH)₂_D₃生物样品含量测定方法的研究进展

Research progress of methods for the determination of 1α,25-(OH)₂ D₃ biological samples

作者：陈瑞, 徐智儒, 刘莉

作者(英文)：

单位：1. 中国医药工业研究总院药理评价研究中心; 上海 200040;2. 上海市生物物质成药性评价专业技术服务中心; 上海 200437

单位(英文)：1. Center for Pharmacological Evaluation and Research, China State Institute of Pharmaceutical Industry, Shanghai 200040, China;
2. Shanghai Professional and Techniical Service Center for Biological Material Druggability Evaluation, Shanghai 200437, China

关键词：vitamin D metabolites; 1α, 25-dihydroxyvitamin D3(1α, 25-(OH)₂ D₃); calcitriol; biological sample; immunoassay; chromatographic method; liquid chromatography-tandem mass spectrometry

关键词(英文)：vitamin D metabolites; 1α, 25-dihydroxyvitamin D3(1α, 25-(OH)₂ D₃); calcitriol; biological sample; immunoassay; chromatographic method; liquid chromatography-tandem mass spectrometry

分类号：R917

出版年·卷·期（页码）：2017,37 (8):1357-1362

DOI: 10.16155/j.0254-1793.2017.01.01

-----摘要：-------------------------------------------------------------------------------------------

1α，25-二羟基维生素D3（1α，25-（OH）₂ D₃）是维生素D体内代谢的重要活性物质，被证实与多种疾病的生理病理进程相关。操作简单且高灵敏度的1α，25-（OH）₂ D₃生物样品含量测定方法的开发有很高的科研和临床应用价值。本综述主要讨论免疫分析法和色谱分析法在1α，25-（OH）₂ D₃生物样品测定中的应用，重点阐述液相色谱-串联质谱联用技术在该领域的应用。

-----英文摘要：---------------------------------------------------------------------------------------

1α,25-dihydroxyvitamin D3(1α, 25-(OH)₂ D₃)is the most important metabolites of vitamin D in vivo,and has been proved to be related to the pathophysiology of various diseases. The development of a simple, highly sensitive 1α,25-(OH)₂ D₃ method for the determination of biological samples has high value in research and clinical application. In this review,we discuss the applications of immunoassay and chromatographic methods for the determination of 1α, 25-(OH)₂ D₃ biological samples and mainly illustrate the applications of liquid chromatography-tandem mass spectrometry in this field.

-----参考文献：---------------------------------------------------------------------------------------

1α,25-(OH)2_D3生物样品含量测定方法的研究进展

Research progress of methods for the determination of 1α,25-(OH)2 D3 biological samples

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1α,25-(OH)₂_D₃生物样品含量测定方法的研究进展

Research progress of methods for the determination of 1α,25-(OH)₂ D₃ biological samples