UPLC-PDA同时测定三黄方药液10种成分含量

目的：建立UPLC-PDA法同时测定三黄方药液中黄柏碱、黄芩苷、小檗碱、黄芩素、芦荟大黄素、汉黄芩素、大黄酸、大黄素、大黄酚、大黄素甲醚10种成分的含量。方法：采用Waters ACQUITY BEH C₁₈（2.1 mm×100 mm，1.7 μm）色谱柱，以乙腈-0.1%磷酸水溶液为流动相，梯度洗脱，流速0.3 mL·min^-1，柱温30℃，检测波长284 nm。结果：在一定浓度范围内，三黄方药液中10种成分均呈良好的线性关系（r>0.999）；平均加样回收率为96.73%～101.7%（RSD<3%，n=6）。3批样品中黄柏碱、黄芩苷、小檗碱、黄芩素、芦荟大黄素、汉黄芩素、大黄酸、大黄素、大黄酚、大黄素甲醚含量分别在0.226～0.233、5.85～6.00、1.92～2.00、0.140～0.151、0.031 4～0.037 5、0.043 6～0.044 7、0.081 5～0.083 1、0.032 8～0.040 6、0.102～0.105、0.340～0.350 mg·mL^-1。结论：经方法学验证，本法可用于三黄方药液的质量控制。

Objective: To establish a UPLC-PDA method for simultaneous determination of ten ingredients, including phellodendrine,baicalin,berberine,baicalein,aloe-emodin,wogonin,rhein,emodin,chrysophanol and physcion,in Sanhuangfang ethanol extract.Methods: The chromatographic analysis was carried out on an ACQUITY BEH C₁₈ column(2.1 mm×100 mm,1.7 μm).The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution with gradient elution at a flow rate of 0.3 mL·min^-1.The column temperature was 30℃,and the detection wavelengths were set at 284 nm.Results: Within a certain concentration range,the ten ingredients all showed good linear relationship(r>0.999) and the average recoveries were with the range of 96.73%-101.7% (RSD<3%,n=6).The contents of phellodendrine,baicalin,berberine,baicalein,aloe-emodin,wogonin,rhein, emodin,chrysophanol and physcion in 3 samples were 0.226-0.233,5.85-6.00,1.92-2.00,0.140-0.151, 0.031 4-0.037 5,0.043 6-0.044 7,0.081 5-0.083 1,0.032 8-0.040 6,0.102-0.105,and 0.340-0.350 mg·mL^-1, respectively.Conclusion: The established method can be used for the quality control of Sanhuangfang ethanol extract.