牛黄清心丸(局方)中辅料蜂蜜的质量评价方法研究

目的: 采用HPLC-ELSD法测定牛黄清心丸(局方)中果糖、葡萄糖、蔗糖和麦芽糖的含量,建立含29味药材的大处方成药中辅料蜂蜜的质量评价方法。方法: HPLC分析使用Prevail Carbohydrate ES(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-水(85:15)为流动相,流速1.0 mL·min^-1,ELSD检测;以果糖、葡萄糖的总量、麦芽糖含量、果糖与葡萄糖含量比值为指标判断牛黄清心丸(局方)中辅料蜂蜜的质量。结果: 果糖、葡萄糖、蔗糖和麦芽糖4个成分有良好的线性关系,精密度、稳定性、重复性的RSD均低于3%,回收率(n=6)分别为100.7%、100.4%、100.1%、100.5%;9个厂家的31批样品有2批麦芽糖含量高出上限,其中1批果糖与葡萄糖含量比值严重偏低,存在使用劣质蜂蜜的情况。结论: 本方法简便、准确,可用于评价大处方成药牛黄清心丸(局方)中辅料蜂蜜的质量。

Objective: To adopt a HPLC-ELSD method to determine fructose, glucose, sucrose and maltose in Niuhuang Qingxin(Jufang)so as to establish a reliable quality evaluation method for honey in complex Chinese patent medicine composed of 29 herbs.Methods: HPLC assay was performed on Prevail carbohydrate ES column(250 mm×4.6 mm, 5 μm)with a mixture of acetonitrile and water(85:15)as the mobile phase at a flow rate of 1 mL·min^-1.The ELSD was used.The total content of fructose and glucose, the maltose content, and the fructose-glucose ratio were considered as quality indexes.Results: All the 4 components showed good linearity.The RSDs of the precision, reproducibility and stability tests were all less than 3%.The average recovery was 100.7%, 100.4%, 100.1% and 100.5%, respectively.The maltose content of 2 in 31 batches exceeded the upper limit, among which the fructose-glucose ratio of 1 batch was much lower than the limit.The lower ratio implied that honey of poor quality was used in these factories.Conclusion: The proposed method is simple and accurate for quality evaluation of honey in the complex Chinese patent medicine Niuhuang Qinxin(Jufang)pills.

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