HPLC同时检测姜黄素-胡椒碱复方自微乳中姜黄素和胡椒碱的含量

目的: 建立在同一色谱条件下同时检测姜黄素-胡椒碱复方自微乳中姜黄素和胡椒碱含量的HPLC含量测定方法. 方法: 采用Purospher STAR LP C₁₈色谱柱(4.6 mm×250 mm,5 μm),以乙腈-4%醋酸水溶液(55:45)为流动相,流速1 mL·min^-1,检测波长342 nm,柱温30 ℃,进样量5 μL. 结果: 姜黄素和胡椒碱浓度分别在20.2~80.8 μg·mL^-1和0.408~1.632 μg·mL^-1范围内线性关系良好;低、中、高3种浓度的平均加样回收率(n=9)分别为98.68%、98.99%、99.38%和98.40%、98.79%、99.48%,RSD分别为1.2%、0.66%、0.79%和0.69%、0.25%、0.95%;以标示量计算的平均含量分别为(43.40±0.55)mg·g^-1和(0.767±0.004)mg·g^-1. 结论: 经方法学验证,该方法是控制姜黄素-胡椒碱复方制剂内在质量的理想方法,适合复方自微乳中姜黄素和胡椒碱的含量测定.

Objective: To establish an HPLC method for the simultaneous determination of the contents of curcumin and piperine in curcumin-piperine compound self-microemulsifying drug delivery system(Cur-PIP-SMEDDS). Methods: The determination was performed on a Purospher STAR LP C₁₈ column(4.6 mm×250 mm,5 μm)with a mobile phase of acetonitrile-4% acetic acid(55:45). The process was carried out with the flow rate of 1.0 mL·min^-1,the wavelength at 342 nm and the column temperature at 30 ℃. A 5 μL aliquot was injected into HPLC for analysis. Results: Good linearities of curcumin and piperine were achieved in the concentration ranges of 20.2-80.8 μg·mL^-1 and 0.408-1.632 μg·mL^-1,respectively. The average recoveries(n=9)of curcumin and piperine were 98.68%,98.99%,99.38% and 98.40%,98.79%,99.48%,respectively,and the relative standard deviations were 1.2%,0.66%,0.79% and 0.69%,0.25%,0.95%,respectively,at three different levels of concentrations. The mean contents of curcumin and piperine were(43.40±0.55)mg·g^-1 and(0.767±0.004)mg·g^-1,respectively. Conclusion: The determination method is proved to be ideal to control the inner quality of Cur-PIP- SMEDDS by methodological verification and suitable for content determination of curcumin and piperine in Cur-PIP- SMEDDS.