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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC法建立人参糖肽注射液特征图谱的研究

Study on establishing fingerprint chromatogram of Ginseng glycopeptide injection by HPLC

分类号:
出版年·卷·期(页码):2014,34 (2):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立人参糖肽注射液特征图谱。方法:通过对人参糖肽注射液中多糖1-苯基-3-甲基-5-吡唑啉酮柱前衍生化,采用高效液相色谱法进行组成糖分析。结果:人参糖肽注射液特定特征图谱由6个共有峰组成,为甘露糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖。结论:高效液相色谱法建立多糖的特征图谱,操作方法简便,分离度高,重复性及稳定性良好,该结果可有效控制人参糖肽注射液的内在质量,同时可作为酸性杂多糖组成糖的测定方法。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish a specific fingerprint of Ginseng glycopeptide injection. Methods: The monosaccharide composition of Ginseng glycopeptide injection was determined by HPLC after preparing pre-column derivatization as 1-phenyl-3-methyl-5-pyrazolone derivant. Results: The specific fingerprint chromatogram of Ginseng glglopeptide injection was composed of 6 common peaks from the fingerprint which were mannose,glucuronic acid,galacturonic acid,glucose,galactose,and arabinose,respectively,which composed the specific fingerprint chromatogram of Ginseng glycopeptide injection. Conclusions: HPLC is a simple,rapid,sensitive,and convenient method with high reproducibility and stability,which can be applied to the quality control of Ginseng glycopeptide injection.And it also can be used to determine the monosaccharide composition of acidic heteropolysaccharides.

-----参考文献:---------------------------------------------------------------------------------------
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[8] YAN Jun (颜军), GUO Xiao-qiang (郭晓强), WU Xiao-yong (邬晓勇), et al.Analysis of monosacchaaride composition in Tremella polysaccharids by non-derivatization high performance liquid chromatography (非衍生化HPLC法分析银耳多糖中单糖组成的初步研究)[J].Food Sci(食品科学), 2007, 28(7):446
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[12] ZHOU Ting-ting (周婷婷), JIANG Xiang-zhi (姜翔之), WANG Ying (王颖), et al.Study on characteristic spectrum of the extract of Marasmius Androsaceus(安络小皮伞提取物特征图谱研究)[J].China Pharm(中国药师), 2013, 16(3):325
[13] HAN Wei (韩威), JIANG Rui-zhi (姜瑞芝), CHEN Ying-hong (陈英红), et a1.Comparison of three chromatographic methods for monosaccharide composition analysis of Tremella polysaccharide(银耳多糖单糖组成分析的3种色谱方法比较)[J].Nat Prod Res Dev(天然产物研究与开发), 2012, 24(3):359

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