HPLC测定不同品种怀地黄中地黄苷A、D的含量

目的：建立HPLC法同时测定地黄中地黄苷A、D含量的方法，为怀地黄种质资源的优选和质量控制提供科学依据。方法：采用Dikma Diamonsil C₁₈柱（4.6 mm×250 mm，5 μm），流动相为乙腈-水系统（4：96），流速为1.0 mL·min^-1，柱温为35 ℃，紫外检测器检测波长为203 nm。结果：13批怀地黄中，鲜地黄中地黄苷A、D的含量分别为0.0955%~0.3916%、0.3609%~0.8955%；生地黄中地黄苷A、D的含量分别为0.1333%~0.5542%、0.1985%~0.6786%，不同品种和产地含量差别较大。结论：本文建立的方法所测数据可作为怀地黄的种资资源优选和质量控制的数据。

Objective: To establish an HPLC method for the determination of rehmanniosides A and D in the roots of Rehmannia glutinosa Libosch.,and to provide scientific reference for the optimization of germplasm resources and quality control of Rehmannia glutinosa Libosch.. Methods: The chromatographic separation was performed on Dikma Diamonsil C₁₈ column(4.6 mm×250 mm,5 μm),a mobile phase of acetonitrile-water(4:96) was used,and the flow-rate was 1 mL·min^-1,and the column temperature was set at 35 ℃,a UV detector was applied with the wavelength of 205 nm. Results: There were big differences in different varieties and origins,the contents of rehmaionosides A and D in fresh rehmannia root were 0.0955%-0.3916%and 0.3609%-0.8955%,and in unprocessed rehmannia root were 0.1333%-0.5542%and 0.1985%-0.6786%. Conclusion: This established determination method has high scientificalness,and the obtained data can be used as a basis for the optimization of germplasm resources and quality control of Rehmannia glutinosa Libosch.

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