快速溶剂萃取-液相色谱法测定人参药材中人参皂苷的含量

目的: 建立快速溶剂萃取(ASE)-液相色谱法测定人参药材中人参皂苷Re、Rg₁、Rb₁、Rc、Rb₂、Rd。 方法: 用三氯甲烷脱脂,水饱和正丁醇提取药材中人参皂苷;采用C₁₈柱,乙腈-水为流动相进行梯度洗脱,检测波长为203 nm,柱温为35℃,进行含量测定。 结果: 人参皂苷化合物响应值与进样量呈良好线性关系(r >0.999),测定精密度RSD﹤2.0%。 结论: 本法操作简便、快速,结果准确,可用于人参药材质量控制。

Objective: To establish an accelerated solvent extraction(ASE)-HPLC method to determine ginsenosides Re,Rg₁,Rb₁,Rc,Rb₂,Rd in Ginseng Radix et Rhizoma. Methods: Trichloromethane was used to remove the lipid ingredients and water-saturated butanol solution was adopted to extract the ginsenosides.A C₁₈ column with mobile phase of acetonitrile aqueous solution was used,and the contents of ginsenosides were determined at a wavelength of 203 nm with the column compartment set at 35℃. Results: The response factor of ginsenosides regarding to the different injections was linear,which generated a satisfactory correlation coefficient(r >0.999).The method precision showed a sound coefficient(RSD%<2.0%). Conclusion: The method is simple,fast and accurate,which is suitable for quality control of Ginseng Radix et Rhizoma.

1 LI Tai-ping(李泰平).Research progress on pharmacological activity of ginsenosides(人参皂苷药理活性的研究进展).Biol Teach(生物学教学),2003,28(4):1
2 ChP(中国药典).2010.Vol Ⅰ(一部):8
3 Richter BE,Jones BA,Porter LN,et al.Accelerated solvent extraction:A technique for sample preparation.Anal Chem,1996,68(6):1033
4 WANG Shu-hong(王淑红),XIONG Ying(熊英),WANG Xiang-hong(王祥红).Sample preparation of Erigeron breviscapus (Vant.)Hant.-Mazz.for determination of content by accelerated solvent extraction(加速溶剂萃取法用于灯盏细辛中灯盏乙素的测定).Chin J Pharm Anal(药物分析杂志),2006,26(5):703
5 CHEN-Jian(陈舰),ZHANG Jing-hua(张景华).Determination of carbendazim residue of tea leaflet by ASE-HPLC(快速溶剂萃取-高效液相法测定茶叶中多菌灵残留量).Guangdong Chem Ind(广东化工),2011,38(1):170
6 LIU Bin(刘斌).Simultaneous quantification of seven flavonoids in eagle tea using pressurized liquid extraction and HPLC(加压溶剂提取-HPLC法同时测定老鹰茶中7个黄酮类化合物).Chin J Pharm Anal(药物分析杂志),2010,30(8):1424
7 Wong LJ,Curtis LW.Recent advances in extraction of neutraceuticals from plants.Trends Food Sci Technol,2006,17(6):300
8 GB/T 19649-2006.Determination of 475 pestcides residue in ceramic wheat(粮谷中475种农药残留测定方法)