罗布麻叶类药材中3个黄酮苷成分HPLC定量分析

目的: 建立HPLC法定量分析罗布麻叶类(红麻和白麻)药材中3种黄酮苷类成分。 方法: 采用Elite-ODS(5 μm,150 mm×4.6 mm)色谱柱,以乙腈-水-四氢呋喃-冰醋酸(8: 81: 10: 1)为流动相,流速1.0 mL·min^-1,检测波长258 nm,柱温30℃。 结果: 芦丁、异槲皮苷和槲皮苷线性范围分别为0.026~0.520,0.094~1.880,0.084~1.680 μg;相关系数分别为0.9996,0.9997,0.9990。回收率(n=5)分别为97.5%,98.4%,97.7%;RSD分别为1.9%,1.8%,2.2%。药材红麻和白麻中黄酮苷类成分含量和分布存在差异。 结论: 所建立的方法快捷、灵敏、准确,可作为鉴别和评价罗布麻叶类药材的质量控制方法。

Objective: To establish an HPLC method for the determination of three flavonoid glycosides in Folium Apocyni Veneti and Folium Poacyni. Methods: The analysis was performed on an Elite-ODS(5 μm, 150 mm×4.6 mm) column with the mobile phase consisted of acetonitrile-water-tetrahydrofuran-acetic acid(8: 81: 10: 1) at a flow rate of 1.0 mL·min^-1, the detection wavelength was 258 nm and the column temperature was 30℃. Results: The linear ranges of rutin, isoquercitrin and quercitroside were 0.026-0.520, 0.094-1.880 and 0.084-1.680 μg with the correlation coefficients of 0.9996,0.9997 and 0.9990, respectively. The average recoveries(n=5) were 97.5%, 98.4%, 97.7% with RSDs of 1.9%, 1.8%, 2.2%. Difference in the distributions and contents of isoquercitrin from two herb medicines was also shown from HPLC analysis. Conclusion: This method is simple, sensitive and rapid, which can be used for the quality control of Folium Apocyni Veneti and Folium Poacyni.