秦皮乙素在玻碳电极上的电化学行为及其含量测定

目的: 研究秦皮乙素在玻碳电极(GCE)上的伏安行为,建立测定秦皮乙素含量的一种新的电化学分析方法。 方法: 在 pH 4.5 磷酸盐缓冲溶液中,采用循环伏安法研究秦皮乙素浓度在玻碳电极上的电化学行为,以差示脉冲伏安法对其含量进行测定。 结果: 秦皮乙素溶液在 2.0×10^-7~3.2×10 ^-6 mol·L^-1 范围内呈良好的线性关系,检测限为 2.6×10^-8mol·L^-1,相对标准偏差为 2.5%。 结论: 建立了秦皮乙素的电化学检测方法,本方法灵敏度高,重复性好,用于中药秦皮中秦皮乙素的含量测定,结果满意。

Objective: To study the voltammetric behavior of esculetine on glassy carbon electrode (GCE) and establish a novel method for esculetine determination. Methods: The electrochemical behavior of esculetine with GCE was studied by cyclic voltammetry (CV) in pH 4.5 phosphate buffer solution (PBS), and the content of esculetine was measured by differential pulse voltammetry (DPV). Results: The relationship between oxidation peak current and the concentration of esculetine was linear in the range of 2.0×10^-7-3.2×10^-6 mol·L^-1.The detection limit was 2.6×10^-8 mol·L^-1,with RSD of 2.5%. Conclusion: The method is accurate,rapid and sensitive,which can be used for the determination of esculetine.

1 ChP(中国药典).2010.Vol Ⅰ(一部):254
2 HUANG Yan-ping(黄燕萍).Determination of esculetine in Xiaoyan tablets by HPLC(HPLC法测定消炎片中秦皮乙素的含量).Heilongjiang Med J (黑龙江医药),2009,22 (1):10
3 WANG Hong-lan(王洪兰),ZHU Dan-ni(朱丹妮),YU Bo-yang(余伯阳).HPLC-DAD-ELSD simultaneous determination of aesculein,linarin and polygalasaponin XXI in compound Guazijin granules(复方瓜子金颗粒中秦皮乙素、蒙花苷和polygalasaponin XXI的HPLC-DAD-ELSD法测定). Chin J Pharm Anal (药物分析杂志),2008,28 (5):685
4 Egan DA,Duff C,Jordan L,et al.High performance liquid chromatographic determination of esculetin and daphnetin in Urine and Plasma.Chromatographia,2003,58:649
5 WONG Siu-kay(王兆基),LAU Chau-ming(刘秋铭),SU Xiao-li(粟晓黎),et al.Characterization of Semen Euphorbiae(生千金子鉴别研究).Chin J Pharm Anal(药物分析杂志),2011,31(7):1304
6 PANG Zhi-gong(庞志功),WANG Bao-qi(汪宝琪),ZHANG Han-li(张汉利).Fluorimetric determination of aesculin and aesculetine in Frainus chinensis Roxb.(秦皮中秦皮甲素和秦皮乙素的荧光测定).Chin J Anal Chem (分析化学研究简报),1996,24 (6):703
7 LI Man-xiu(李满秀),ZHAO Er-lao(赵二劳),REN Guang-ming(任光明),et al.Studies on the sensitized fluorescence characteristics of the aesculetin-CTMAB with calcium ion .Chin J Spect Lab (光谱实验室),2003,22(5):787
8 LI Yun(李云).Fluorescence analysis of Chinese herbal medicine-Cortex Fraxini and its effective components(中药秦皮及其有效成分的荧光分析方法研究): .Shijiazhuang(石家庄):Hebei Normal University(河北师范大学硕士学位论文).2007
9 LI Qi-long(李启隆),WANG Qian(汪倩),HU Jing-bo(胡劲波),et al.Study on oscillopolarography of esculetin(秦皮乙素的单扫示波极谱法研究).J Beijing Normal Univ (Nat Sci)(北京师范大学学报(自然科学版)),1996,32 (3):386
10 LI Qun(李群),LU Wei(卢炜),WANG Wen-lan(王文兰),et al.Determination of aesculetin in processed product of Semen Euphorbiae(千金子炮制品中秦皮乙素含量的测定). Chin Tradit Pat Med(中成药),2000,22(2):137
11 ZHANG Qi(张琦),ZHANG Xin-shen(张新申),DAI Yong(代勇).Rapid determination of fraxin,esculin and esculetin in Cortex Fraxini by capillary zone electrophoresis(3种秦皮香豆素的毛细管区带电泳快速分析).Chin Pharm J(中国药学杂志),2009,44(16):1269
12 Laviron E.Adsorption autoinhibition and autocatalysis in polarography and in linerpotential sweep voltammetry. J Elect Chem,1974,52:370