HPLC测定不同产地与不同部位千层塔中石杉碱甲和石杉碱乙含量

目的:测定不同产地、不同部位千层塔药材中石杉碱甲和石杉碱乙的含量。方法:采用UItimate XB-C₁₈(4.6 mm×250 mm,5 μm)色谱柱,流动相为乙腈-甲醇-水(10:55:240),含0.32%磷酸和0.5%三乙胺,流速1.0 mL·min^-1,检测波长309 nm,柱温25℃。结果:测得各地千层塔中石杉碱甲、石杉碱乙最高含量分别为0.0900%和0.0183%,各地石杉碱甲、石杉碱乙最低含量分别为0.0014%和0。结论:所测定的方法简便、可靠、快速,重复性好,结果稳定,适用于千层塔药材中石杉碱甲和石杉碱乙的同时快速含量测定,该研究可为千层塔最佳产地及最佳药用部位的确定提供参考。

Objective:To develop an HPLC method for simultaneous determination of huperzine A(Hup-A) and huperzine B(Hup-B) in different parts of Huperzia serrate from different habitats. Methods:The assay was performed on an UItimate XB-C₁₈(4.6 mm×250 mm,5 μm) column with the mobile phase of acetonitrile-methanol-water(10:55:240) containing 0.32% phosphoric acid and 0.5% triethylamine.The flow rate was 1.0 mL·min^-1,the detection wavelength was set at 309 nm,and the column temperature was set at 25℃. Results:The contents of huperzine A and huperzine B in Huperzia serrata collected at different habitats were evidently different.The maximum contents of huperzine A and huperzine B were 0.0900% and 0.0183%,respectively;the minimum contents of huperzine A and huperzine B were 0.0014% and 0,respectively. Conclusions:The method is simple,accurate and repeatable,which provides a reliable way for evaluating the quality of Huperzia serrata by measuring huperzine A and huperzine B,as well as provide a reference for the determination of the best origin and the most rational part of the plant.