石墨烯-Nafion-纳米金增敏印刷电极伏安法测定青藤碱

目的: 采用石墨烯(GS)-Nafion-纳米金(Au)增敏印刷电极(SPCEs)建立青藤碱(SN)的测定方法,探讨SN在修饰电极上的电化学行为。 方法: 将GS-Nafion溶液涂覆于SPCEs表面制得GS-Nafion︱SPCEs电极,然后于上述电极表面化学镀Au制得Au/GS-Nafion︱SPCEs电极,在B-R缓冲液中,采用循环伏安(CV)和示差脉冲伏安(DPV)法研究SN的电化学性质。 结果: 在优化的实验条件下,DPV氧化峰电流与SN浓度在5.0×10^-7~1.0×10^-4 mol·L^-1 范围内呈良好的线性关系,检出限为2.2×10^-7 mol·L^-1。 结论: 该修饰电极价格低廉,制备容易,样品用量少,抗干扰性强,可用于正清风痛宁注射液中SN含量测定。

Objective: To investigate the electrochemical behavior of sinomenine (SN) by screen-printed carbon electrodes (SPCEs) sensitized by graphene sheets (GS),Nafion and gold nanoparticals (Au). Methods: SPCEs was coated by GS-Nafion solution and Au was painted on its surface to fabricate Au/GS-Nafion︱SPCEs electrode.The electrochemical characteristics of SN were studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) in a Britton-Robinson (B-R) buffer solution. Results: Under optimum experimental conditions,the anodic peak current obtained by DPV was linearly dependent on SN concentration in the range of 5.0×10^-7-1.0×10^-4 mol·L^-1.The detection limit was 2.2×10^-7 mol·L^-1. Conclusion: The modified electrode is inexpensive,easy to prepare with fewer sample volume and strong anti-interference and is suitable for the determination of SN in Zhengqing Fengtongning injection samples.