红毛五加皮中刺五加苷E鉴别与测定及质量标准研究

目的: 建立红毛五加皮中刺五加苷E的定性鉴别和含量测定方法,建立红毛五加皮的质量标准。 方法: 照2010年版中国药典方法检查红毛五加皮中总灰分和酸不溶性灰分,测定其浸出物含量。采用TLC法鉴别刺五加苷E,薄层色谱使用硅胶G薄层板,以三氯甲烷-甲醇-水(6: 3: 1)的下层溶液为展开剂,10%硫酸乙醇溶液为显色剂;采用HPLC法测定刺五加苷E的含量,色谱柱为Welchrom C₁₈(250 mm × 4.6 mm,5 μm),流动相为乙腈-0.05%磷酸(13: 87),流速1 mL·min^-1,检测波长207 nm,柱温35℃。 结果: 红毛五加皮中总灰分平均为6.69%,酸不溶性灰分平均为0.71%,浸出物平均含量为15.25%,刺五加苷E薄层斑点清晰,特征明显。刺五加苷E的线性范围为0.02696~1.0784 μg(r=1.000),平均加样回收率(n=6)为97.2%(RSD＝1.7%)。 结论: 本文建立的薄层鉴别方法特征明晰,液相色谱方法分离良好,经方法学验证,可用于红毛五加皮药材的质量检测。 关键词 :红毛五加皮;刺五加苷E;薄层色谱法;高效液相色谱法;中药材;标准改进

Objective: To establish an identification and determination method of eleutheroside E in Cortex Acanthopanacis Giraldii,and set up the quality standards of Cortex Acanthopanacis Giraldii. Methods: According to 2010 edition of Chinese Pharmacopoeia,the total ash,acid-insoluble ash and extractum were determined.The TLC and HPLC methods were used for qualitative identification and quantitative determination of eleutheroside E.TLC method used silica gel G as the coating substance,underlayer of chloroform-methanol-water(6: 3: 1)as the developing solvent,10% sulfuric acid alcohol solution as coloration.HPLC method was performed on a welchrom C₁₈ column(250 mm × 4.6 mm,5 μm),using the mobile phase of acetonitrile-0.05% phosphonic acid water solution(13: 87)with a flow rate at 1mL·min^-1,the detection wavelength was set at 207 nm and column temperature was 35℃. Results: The average content of total ash and acid-insoluble ash were 6.69% and 0.71%,the extractum content was 15.25% on average.The TLC identification was highly specific and spot of eleutheroside E was clear.The linear range of eleutheroside E was between 0.02696-1.0784 μg(r= 1.000),and the average recovery was 97.2% with RSD 1.7%(n=6). Conclusion: The established TLC and HPLC methods can be used for the quality control of Cortex Acanthopanacis Giraldii.

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