GC法同时测定降香中4个成分的含量

目的: 建立降香挥发油中反式-β-金合欢烯、β-没药烯、反式-橙花叔醇、金合欢醇的气相色谱含量测定方法。 方法: 色谱条件为HP-5 MS(30 m×0.25 mm,0.25 μm)色谱柱,程序升温;进样口温度250 ℃,流速1.0 mL·min^-1;分流比20:1;FID检测器,温度250 ℃;载气:氮气。 结果: 反式-β-金合欢烯、β-没药烯、反式-橙花叔醇、金合欢醇的线性范围分别为0.011~0.212 mg·mL^-1(r=0.9998),0.011~0.659 mg·mL^-1(r=0.9999),0.051~10.200 mg·mL^-1(r=0.9996),0.011~0.222 mg·mL^-1(r=0.9998);加样回收率(n=9)分别为99.5%,98.4%,99.7%,96.6%。 结论: 该方法简便、准确,重复性好,为降香的质量控制提供了依据。

Objective: To develop a method for determination of trans-β-farnesene,β-bisabolene,trans-nerolidol and farnesylalcohol in volatile oil of Dalbergia odorfera by gas chromatography. Methods: The sample was determined with capillary column of HP-5MS(30 m×0.25 mm×0.25 μm),and FID detector with the detector temperature at 250 ℃.The injector temperature was set at 250 ℃,the flow rate was 1.0 mL·min^-1;split injection was conducted with split radio of 20:1.Nitrogen was used as carrier gas. Results: Trans-β-farnesene,β-bisabolene,trans-nerolidol and farnesylalcohol had good linearity in the ranges of 0.011-0.212 mg·mL^-1(r=0.9998),0.011-0.659 mg·mL^-1(r=0.9999),0.051-10.200 mg·mL^-1(r=0.9996),0.011- 0.222 mg·mL^-1(r=0.9998),respectively.The average recoveries(n=9)were 99.5%,98.4%,99.7% and 96.6%,respectively. Conclusion: A rapid,sensitive,accurate and high recovery rate method was developed.This study can provide a scientific basis for the quality control of Dalbergia odorfera.