HPLC同时测定山核桃外蒲壳中球松素和大黄酚含量

目的: 建立同时测定山核桃外蒲壳中球松素和大黄酚含量的分析方法。 方法: 采用HPLC法,色谱柱为Shim-pack C₁₈(250 mm×4.6 mm,5 μm),以甲醇-0.1%磷酸水溶液(79∶21)为流动相,流速0.8 mL·min^-1,检测波长286 nm,柱温25 ℃。 结果: 山核桃外蒲壳中球松素和大黄酚的线性范围分别为4.12~25.7 μg·mL^-1和1.01~13.4 μg·mL^-1,平均加样回收率(n=6)分别为98.8%和98.0%。 结论: 该方法准确可靠、结果稳定、重复性好,为评价山核桃外蒲壳的质量提供依据。

Objective: To establish a method for simultaneous determination of pinostrobin and chrysophanol in the hull of Carya cathayensis. Methods: The analysis was carried out on a Shim-pack C₁₈(250 mm×4.6 mm,5 μm) column eluted with a mobile phase of methanol-water containing 0.1% phosphoric acid(79∶21);The flow rate was 0.8 mL·min^-1 and column temperature was 25 ℃;The detection wavelength was set at 286 nm. Results: The calibration curves of pinostrobin and chrysophanol were linear in the ranges of 4.12-25.7 μg·mL^-1 and 1.01-13.4 μg·mL^-1,and the average recoveries(n=6) were 97.8% and 98.0%,respectively. Conclusion: The method is simple,accurate and sensitive,which can be used as a method of quantitative determination of pinostrobin and chrysophanol in the hull of Carya cathayensis.

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